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172,478 results

Showing: 5021 - 5040 of 172478 results

  5. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  7. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  8. pcDNA3.1.Gα11-LgB97

    Plasmid
    #134361
    Purpose
    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα11-LgB97 (GNA11 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134361-plasmid-map-sequence-id-306399

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  9. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  14. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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Showing: 5021 - 5040 of 172478 results