Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,604 results

Showing: 5021 - 5040 of 177604 results

  1. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104567-plasmid-map-sequence-id-201429

    Enlarge

  3. pCMV-pro-Il1b-C-Flag

    Plasmid
    #75131
    Purpose
    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    interleukin 1 beta (Il1b Mouse)
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    (please see depositor comments below)
    Promoter
    CMV
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    75131-plasmid-map-sequence-id-192693

    Enlarge

  4. pCI-neo Fluc EGFP

    Plasmid
    #90170
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    90170-plasmid-map-sequence-id-226521

    Enlarge

  5. pCI-neo FlucDM EGFP

    Plasmid
    #90172
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Mutation
    R188Q, R261Q in FLuc
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    90172-plasmid-map-sequence-id-226525

    Enlarge

  6. pME-GAL4-ERT2-VP16

    Plasmid
    #82588
    Purpose
    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression
    Depositor
    Kryn Stankunas
    Publication
    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-ERT2-VP16
    Promoter
    None
    Available since
    Dec. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    82588-plasmid-map-sequence-id-161151

    Enlarge

  8. pYFP-RPB1aAmr

    Plasmid
    #75284
    Purpose
    Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells
    Depositor
    Xavier Darzacq, Robert Singer
    Publication
    Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A (POLR2A Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    N792D alpha-amanitin–resistant
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    75284-plasmid-map-sequence-id-496264

    Enlarge

  10. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Publication
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    7 Articles
    74939-plasmid-map-sequence-id-151347

    Enlarge

  16. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    7 Articles
    72268-plasmid-map-sequence-id-134573

    Enlarge

  18. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58694-plasmid-map-sequence-id-90691

    Enlarge

  19. GFP_CD47_LU

    Plasmid
    #65474
    Purpose
    GFP tagged CD47 with its own long 3'UTR
    Depositor
    Christine Mayr
    Publication
    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    Expression
    Mammalian
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65474-plasmid-map-sequence-id-117297

    Enlarge

  20. GFP-PBRM1

    Plasmid
    #65387
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65387-plasmid-map-sequence-id-322048

    Enlarge

Showing: 5021 - 5040 of 177604 results