We narrowed to 12,325 results for: NSI

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EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191184

Purpose

Expression of RPLP1 sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:RPLP1.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

Plasmid

#191175

Purpose

Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (frameshifted)

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pZH512

Plasmid

#102666

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-MB

Plasmid

#31703

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-106a-363

Use

Retroviral

Expression

Mammalian

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti_dNmeCas9

Plasmid

#120077

Purpose

dNmeCas9 with no sgRNA cassette lenti construct

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

dNmeCas9

Use

Lentiviral

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pL59-Nat

Plasmid

#140465

Purpose

CRISPR/Cas9 engineering with nourseothricin selection

Depositor

Gianni Liti

Article

Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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IFI16-(515-710)-HinB

Plasmid

#35053

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(515-710)-HinB (IFI16 Human)

Tags

His

Expression

Bacterial

Mutation

Contains HinB domain: aa 515-710

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
pAB035a

Plasmid

#79190

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-SH2ABL1

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAID1.2-CMV-NGFP-mAID

Plasmid

#140605

Purpose

Expression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-4

Plasmid

#197694

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

CmYLCV promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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SERA5-bio-His

Plasmid

#50817

Purpose

Expresses enzymatically monobiotinylated full length SERA5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3_AKAR5Δ

Plasmid

#119912

Purpose

Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

AKAR5Δ

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-1002

Plasmid

#31711

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-302b, c, a, d

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pHR-MCS-YFP-NLS

Plasmid

#145274

Purpose

Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MCS and YFP

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCA-ATG-intron-tdT3Myc

Plasmid

#36883

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Tags

3 Myc tags

Expression

Mammalian

Mutation

beta-globin intron containing a loxP site inserte…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA2-DNzf

Plasmid

#1291

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Mutation

S263G and M434V

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
MSRP5-bio-His

Plasmid

#50805

Purpose

Expresses enzymatically monobiotinylated full length MSRP5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSRP5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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G273D HA-dynamin 1 pUHD10-3

Plasmid

#34689

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1 Human)

Tags

HA (variant)

Expression

Mammalian

Mutation

G273D

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only