We narrowed to 8,115 results for: gnal

1. pcDNA4/TO/GFPZNF598-9P-9A

   Plasmid

   #141192

   Purpose

   Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   ZNF598 (ZNF598 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   all 3 proline repeats are mutated to Alanine (9xP…

   Promoter

   CMV

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GET1_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176985

   Purpose

   Mammalian expression vector encoding GET1 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GET1 (GET1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMP108 Nanog-Venus-2a-mCherry

   Plasmid

   #159743

   Purpose

   Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

   Depositor

   Sergi Regot

   Article

   Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

   Insert

   Nanog homeobox (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   Venus-2a-mCherry

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xNLS-NLP-cMyc-cMyc AspCas12a

   Plasmid

   #182122

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc AspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223-GOLPH3

   Plasmid

   #21688

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Golgi Phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. LIC-Z-YFP

   Plasmid

   #153544

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   Venus YFP

   Expression

   Mammalian

   Mutation

   with mCherry in LIC-Z replaced by YFP

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-HA-CAD S1900A

   Plasmid

   #46240

   Purpose

   FLAG-HA-CAD with S1900 mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1900 changed to Alanine (S1900A)

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

   Plasmid

   #181842

   Purpose

   EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains only residues 12-112 of PKA RIα.

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RCAS-sgATG7

   Plasmid

   #75228

   Purpose

   An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

   Depositor

   Noor Gammoh

   Article

   Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

10. EBFP2-UtrCH

    Plasmid

    #124656

    Purpose

    F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Utrophin (UTRN Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPGK-T7/2-CD44v8-10 (-cyt)

    Plasmid

    #137822

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v8-10 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LP725

    Plasmid

    #90182

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 561-1826

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa residues 561-1826

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LP399

    Plasmid

    #90176

    Purpose

    pEGFP-C1 with cDNA encoding HsKIF13B aa residues 1-1289

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13B (KIF13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    aa residues 1-1289

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FLAG-hDIXDC1 S592A

    Plasmid

    #61223

    Purpose

    Mammalian expression vector encoding long isoform of human DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    human DIXDC1 (DIXDC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 592 to Alanine

    Promoter

    CMV

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRK5-FLAG-FLCN (C terminus)

    Plasmid

    #72296

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FLCN C-terminus (FLCN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains only C terminus of FLCN as defined in PM…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pET28A-p53 (1-73) (P to A)

    Plasmid

    #62069

    Purpose

    Expression of human p53 (1-73) (all P to A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; all Prolines changed …

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P1-HA-MEK1 C121S

    Plasmid

    #53164

    Purpose

    Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    C121S

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only