We narrowed to 164,313 results for: HTT

1. pKT-PDGFRa-IRES-Katushka

   Plasmid

   #133081

   Purpose

   Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Insert

   PDGFRa (Pdgfra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMN234

   Plasmid

   #32108

   Depositor

   Michael Niederweis

   Article

   Stephan et al Gene. 2004 Dec 8;343(1):181-90.

   Insert

   flpe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   imyc

   Available Since

   Nov. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSheLL

   Plasmid

   #37319

   Depositor

   John Schiller

   Article

   Buck et al J Virol. 2004 Jan;78(2):751-7.

   Insert

   BPVL1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PA-mCherry1-PDHA1-N-10

   Plasmid

   #57193

   Purpose

   Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   PDHA1 (PDHA1 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPD708 DsRed-Express2-ZF21

   Plasmid

   #138795

   Purpose

   Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.

   Depositor

   Joshua Leonard

   Insert

   DsRed-Express2-ZF21
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJM104: pTrc RFP on pCM66T

   Plasmid

   #74745

   Purpose

   pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pTrc

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R777-E151 Hs.PAK3

   Plasmid

   #70435

   Purpose

   Gateway ORF clone of human PAK3 [NM_002578.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   PAK3 (PAK3 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRL-TK CXCR4 6x

   Plasmid

   #11308

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

   Insert

   6 bulged bind sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-syn-axon-jGCaMP8f-WPRE

   Plasmid

   #186041

   Purpose

   jGCaMP8f expression in axons under the control of the synapsin promoter

   Depositor

   Loren Looger

   Insert

   axon-jGCaMP8f
   

   Use

   AAV

   Tags

   GAP43-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-syn-LifeAct-jGCaMP8m-WPRE

    Plasmid

    #186039

    Purpose

    AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter

    Depositor

    Loren Looger

    Insert

    LifeAct-jGCaMP8m
    

    Use

    AAV

    Tags

    LifeAct-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. WT-long-UBASH3A-V5-6xHis

    Plasmid

    #192101

    Purpose

    Mammalian expression of human wild-type long-form UBASH3A with V5-6xHis tag

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RAB30

    Plasmid

    #25542

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB30 (RAB30 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBMTBX-1

    Plasmid

    #26072

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-MK2

    Plasmid

    #30187

    Depositor

    Bruce Tempel

    Insert

    MK2 (Kcna2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pMGAHisTev5B

    Plasmid

    #37221

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF5B
    

    Use

    Tags

    His

    Expression

    Mutation

    N-term truncation (contains aa 396-1002)

    Promoter

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

19. T7-U4

    Plasmid

    #111336

    Purpose

    for in vitro snRNA transcription

    Depositor

    Christine Guthrie

    Article

    Ghetti et al RNA. 1995 Apr;1(2):132-45.

    Insert

    snR14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmG3BP_G

    Plasmid

    #146349

    Purpose

    Insect Expression of DmG3BP

    Depositor

    Elisa Izaurralde

    Insert

    DmG3BP (rin Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V632G compared to AF231031.1, exact match to NP_5…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZHY051

    Plasmid

    #44987

    Depositor

    Daniel Voytas

    Article

    Zhang et al Plant Physiol. 2012 Nov 2.

    Type

    Empty backbone

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pINER5

    Plasmid

    #185895

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 25, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pHH0103 NEDD4 WW domain #1

    Plasmid

    #104208

    Purpose

    Bacterial expression of WW domain #1 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. DUSP7

    Plasmid

    #27976

    Depositor

    Igor Astsaturov

    Insert

    DUSP7 (DUSP7 Human)
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

27. mTagRFP-T-Rab4a-7

    Plasmid

    #58025

    Purpose

    Localization: Ras GTPase, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. LHP398 - Ub-K48R/K63R

    Plasmid

    #32167

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K48R and K63R

    Promoter

    CUP1

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pTYB2-eIF1

    Plasmid

    #37217

    Depositor

    Jon Lorsch

    Article

    Acker et al Methods Enzymol. 2007;430:111-45.

    Insert

    eIF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pflaG

    Plasmid

    #45477

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    flaA

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pX551-miniCMV-CjCas9

    Plasmid

    #107033

    Purpose

    AAV plasmid expressing CjCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NM-01

    Plasmid

    #69799

    Purpose

    Bacterial expression of Cas9 with His, MBP, and Strep tags

    Depositor

    Gerard Cagney

    Insert

    Cas9
    

    Use

    Tags

    HIS, MBP, and StrepII

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

33. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAd:TRE-gateway

    Plasmid

    #30217

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RAB2B

    Plasmid

    #25534

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB2B (RAB2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid

    #191529

    Purpose

    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    H2BK_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHH0103 NEDD4 WW domain #4

    Plasmid

    #104211

    Purpose

    Bacterial expression of WW domain #4 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #4 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge