We narrowed to 151,802 results for: DES

1. pENTR223-BDLF1

   Plasmid

   #195794

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BDLF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BDLF1
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT9-AAGAB

   Plasmid

   #198332

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AAGAB (AAGAB Human)
   

   Use

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

   Plasmid

   #184645

   Purpose

   Retroviral expression of BirA Rap1 I312R

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   BirA-rap1-I312R
   

   Use

   Retroviral

   Tags

   BirA FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSunS 1-335

   Plasmid

   #171673

   Purpose

   SunS 1-335 expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pROS13_IDH2 #7

   Plasmid

   #166102

   Purpose

   This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH2 sgRNA #7 (IDH2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pROS13_IDH1 #4

   Plasmid

   #166101

   Purpose

   This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH1 sgRNA #4 (IDH1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Fah_S

   Plasmid

   #163086

   Purpose

   Swingshift mutant FAH in mammalian cells

   Depositor

   Joseph Takahashi

   Article

   Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

   Inserts

   Fah_S
   Fah_S
   

   Use

   Tags

   Myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-P1-N-mCherry-C

   Plasmid

   #159437

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   mCherry
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. YCplac33-NAT_NDT1

   Plasmid

   #140593

   Purpose

   Express NDT1 in S. cerevisiae

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   NDT1 promoter and 3'UTR

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET_3H1L_02.395

    Plasmid

    #155198

    Purpose

    Expresses 3H1L_02.395 in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3H1L_02.395
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ_NAND4_L17_S24_S11_S19_S13

    Plasmid

    #132732

    Purpose

    T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND4_L17_S24_S11_S19_S13
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG_ToeRep_N09_switch

    Plasmid

    #132741

    Purpose

    T7 RNAP-driven expression of first-generation toehold repressor index 9 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRep_N09_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG_Ptet*_ToeRep_N01_switch

    Plasmid

    #132737

    Purpose

    Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    Ptet*_ToeRep_N01_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK_ToeRepG2_N19_switch

    Plasmid

    #132744

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK_ToeRepG2_N64_switch

    Plasmid

    #132743

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N64_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYZ_Ptet*_3WJrep_N19_switch

    Plasmid

    #132739

    Purpose

    Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    Ptet*_3WJrep_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ_NAND3_L11_S11_S13_S19

    Plasmid

    #132731

    Purpose

    T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_NAND3_L11_S11_S13_S19
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-OSF-TRIM5-21R(1-300)

    Plasmid

    #79029

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5-21R (1-300)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-OSF-TRIMCyp (owl monkey)

    Plasmid

    #79035

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH003

    Plasmid

    #80397

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold - Venus YFP
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    Fused by GS linker to LZx domain and Venus has N-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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