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We narrowed to 10,769 results for: ESP

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Showing: 7621 - 7650 of 10769 results

  1. Spike Display_Part 3 DO

    Plasmid
    #172723
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 3 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172723-plasmid-map-sequence-id-339286

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  2. Spike Display_Part-4 DO

    Plasmid
    #172724
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 4 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    172724-plasmid-map-sequence-id-339287

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  3. pLEX307_CR-PDHA2S293A

    Plasmid
    #115204
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115204-plasmid-map-sequence-id-231794

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  4. pLEX307_CR-PDHA2S291A/S293A

    Plasmid
    #115205
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115205-plasmid-map-sequence-id-232427

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  5. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid
    #115187
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115187-plasmid-map-sequence-id-225414

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  6. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid
    #115188
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115188-plasmid-map-sequence-id-225424

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  7. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid
    #115189
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115189-plasmid-map-sequence-id-225429

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  8. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid
    #115190
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115190-plasmid-map-sequence-id-225431

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  9. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid
    #115191
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115191-plasmid-map-sequence-id-225432

    Enlarge

  10. pLEX307_CR-PDHA2

    Plasmid
    #115199
    Purpose
    Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115199-plasmid-map-sequence-id-232425

    Enlarge

  11. pLEX307_CR-PDHA2S291A

    Plasmid
    #115203
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115203-plasmid-map-sequence-id-232429

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  19. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  21. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  23. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available Since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  27. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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Showing: 7621 - 7650 of 10769 results