We narrowed to 19,449 results for: tec

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pconst #1-RFP

Plasmid

#81075

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23119

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB6677

Plasmid

#106155

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-PD-4xDQNAT

Plasmid

#128391

Purpose

In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Protein D

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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PibpA GFP pZa

Plasmid

#170076

Purpose

GFP expression under the control of E. coli ibpA promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

PibpA

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PfxsA GFP pZa

Plasmid

#170075

Purpose

GFP expression under the control of E. coli fxsA promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

PfxsA

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.CREB.WPRE

Plasmid

#38050

Depositor

James Uney

Article

Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

Insert

CREB (Creb1 Rat)

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pFOX6-Tier1-PhCMV-TdTomato

Plasmid

#169515

Purpose

Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven dimeric red fluorescent protein TdTomato

Expression

Mammalian

Promoter

PhCMV

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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LR (bact: HyPer)

Plasmid

#162069

Purpose

Used for the diffuse visualization of H2O2 production in cells.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

beta actin 2 (actb2 Zebrafish)

Use

Gateway donor vector

Tags

HyPer

Promoter

T3

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUCBB-ptet-eGFP

Plasmid

#32554

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

ptet

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pOTF3-AK (clone3)

Plasmid

#21165

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

POUF5F1 (genomic DNA) (POU5F1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

NeoR, EGFP, and SV40 polyA are between the two ge…

Available Since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23107

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15Ω1

Plasmid

#101710

Purpose

GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pU6nH1-HD10kb

Plasmid

#172657

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD10kbA/pegRNA-HD10kbB

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFPL-Yh2

Plasmid

#61650

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
TAL2274

Plasmid

#36711

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Left (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pUCBB-pBAD-eGFP

Plasmid

#32553

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

pBAD

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-FLAGLICNot

Plasmid

#140184

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

FLAG

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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