We narrowed to 7,101 results for: tev

1. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

   Plasmid

   #175020

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   D/bovine/Nebraska/9-5/2012
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   s57a esterase knock out mutant

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLAP-BUBR1

   Plasmid

   #114052

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1 (BUB1B Human)
   

   Use

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance)

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. (SUMO)10-(SIM)5

   Plasmid

   #126866

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_human-pro-IL-18_DeltaN24

   Plasmid

   #214327

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Deletion of first 24 amino acids

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAD16_CAD_12-490_pvp008

   Plasmid

   #124842

   Purpose

   Expresses Aspergillus terreus CAD in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-11, synthetic codon optimiz…

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNH-TrxT-G-S18

   Plasmid

   #127827

   Purpose

   Production of recombinant human ribosomal protein S18

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS18 (RPS18 Human)
   

   Use

   Tags

   His6 - Thioredoxin - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWzyE-distrepII

   Plasmid

   #196667

   Purpose

   Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF11-SFPQ(276-598)

   Plasmid

   #135437

   Purpose

   Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   Splicing Factor Proline And Glutamine Rich (SFPQ Human)
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS-45

    Plasmid

    #40586

    Depositor

    Harald Schwalbe

    Article

    Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14

    Insert

    yTAND12-TEV-hTBPc (TAF1, TBP Budding Yeast, Human)
    

    Use

    Tags

    His-tag and yTAND12

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Use

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. gZiPro

    Plasmid

    #198441

    Purpose

    Activity/inhibition assays, 19F-NMR

    Depositor

    Ute Hellmich, Tanja Schirmeister

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    glycine linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    R95*A,

    Promoter

    T7 promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZ-mouseGIRK2-eGFP-StII-6xHis

    Plasmid

    #124277

    Purpose

    Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Use

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG8Ha-SERBP1(149-400)

    Plasmid

    #176516

    Purpose

    Expresses the human C-terminal SERBP1 149-400 construct.

    Depositor

    David Libich

    Article

    Baudin et al Front Mol Biosci. 2021 Sep 24;8:744707.

    Insert

    SERBP1 149-400 (SERBP1 Human)
    

    Use

    Tags

    His8 tag, linker, TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-148 and 401-408

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-22HT-MSI2(8-193)

    Plasmid

    #60356

    Purpose

    Express the RBD of human MSI2 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    human MSI2 (8-193) (MSI2 Human)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 8-193 of MSI2

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

    Plasmid

    #124278

    Purpose

    Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Use

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    R201A

    Promoter

    AOX1

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. human TLNRD1 pET151

    Plasmid

    #159384

    Purpose

    Expresses human TLNRD1 in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLAP-BUBR1-dKARD

    Plasmid

    #114053

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-dKARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF11-SFPQ(214-598)

    Plasmid

    #135440

    Purpose

    Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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