Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 64,615 results for: cell;

Showing: 10201 - 10240 of 64615 results

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SITS-mCherry-Bin1a

Plasmid

#176024

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

mCherry

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.25_S6

Plasmid

#173960

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 6 (S6) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.35_S1

Plasmid

#173954

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 1 (S1) from SOX9 enhancer cluster EC1.35

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.35_S1-2

Plasmid

#173953

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer regions 1 and 2 (S1 and S2) from SOX9 enhancer cluster EC1.35

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del12

Plasmid

#173984

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #12

Use

Luciferase

Mutation

Tiled deletion #12

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.25_S5

Plasmid

#173959

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 5 (S5) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.25_S4

Plasmid

#173958

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 4 (S4) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak2

Plasmid

#173948

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 2 from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del3

Plasmid

#173975

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #3

Use

Luciferase

Mutation

Tiled deletion #3

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del6

Plasmid

#173978

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #6

Use

Luciferase

Mutation

Tiled deletion #6

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del4

Plasmid

#173976

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #4

Use

Luciferase

Mutation

Tiled deletion #4

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del1

Plasmid

#173973

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #1

Use

Luciferase

Mutation

Tiled deletion #1

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del10

Plasmid

#173982

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #10

Use

Luciferase

Mutation

Tiled deletion #10

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del9

Plasmid

#173981

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #9

Use

Luciferase

Mutation

Tiled deletion #9

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del11

Plasmid

#173983

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #11

Use

Luciferase

Mutation

Tiled deletion #11

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_min2

Plasmid

#173951

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del2

Plasmid

#173974

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #2

Use

Luciferase

Mutation

Tiled deletion #2

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_min1

Plasmid

#173950

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer region 1 (min1) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del5

Plasmid

#173977

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #5

Use

Luciferase

Mutation

Tiled deletion #5

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2

Plasmid

#173949

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.35_S2

Plasmid

#173955

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 2 (S2) from EC1.35 SOX9 enhancer cluster EC1.35

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del7

Plasmid

#173979

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #7

Use

Luciferase

Mutation

Tiled deletion #7

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.25_S3

Plasmid

#173957

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 3 (S3) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.45_p300peak1-2_del8

Plasmid

#173980

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #8

Use

Luciferase

Mutation

Tiled deletion #8

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGK-Ldb3-mKate2

Plasmid

#128766

Purpose

Fluorescent mKate reporter for Ldb3

Depositor

Deepak Srivastava

Article

Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

Insert

Ldb3 promoter (Ldb3 Mouse)

Expression

Mammalian

Promoter

Ldb3

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-Lyn-AktAR2

Plasmid

#125197

Purpose

SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

Lyn-AktAR2

Use

Transposon

Tags

Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Expression

Plant

Promoter

RCH1

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
pmTHC_PGKneoLox2DTA.2

Plasmid

#112624

Purpose

H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-mTFP1 (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

mTeal (mTFP1)

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEHC_PGKneoLox2DTA.2

Plasmid

#112623

Purpose

H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Emerald

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Article

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaster1

Plasmid

#58525

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 )
myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
Nanog (NANOG Human)
IRES

Promoter

CAG and EF1a

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

Plasmid

#193349

Purpose

tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-G3BP1-WT

Plasmid

#135997

Purpose

WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcVpx.myc

Plasmid

#213388

Purpose

Generate lentiviral vector which increases gene delivery into primary human myeloid cells

Depositor

Nathaniel Landau

Article

Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

Insert

SIVmac vpx (vpx Simian Immunodeficiency Virus)

Use

Lentiviral

Tags

myc-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only
pFX-Tom20-EGFP

Plasmid

#174186

Purpose

Express EGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-mCherry-Rab11

Plasmid

#174461

Purpose

Express mCherry with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-mito-EYFP

Plasmid

#174182

Purpose

Express EYFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EYFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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