We narrowed to 25,569 results for: promoter

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ135-tRNA2.0

Plasmid

#158397

Purpose

Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pmGFP10C-Tau

Plasmid

#71433

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Tags

GFP (1-212)

Expression

Mammalian

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 p53

Plasmid

#59360

Purpose

Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

shRNA p53 (mouse) (Trp53 Mouse)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL Blast mouse E-cadherin

Plasmid

#18804

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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ST-GFP

Plasmid

#162500

Purpose

Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

ST6Gal1 (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBS U6a gRNA

Plasmid

#158961

Purpose

gRNA backbone driven by zebrafish U6a promoter

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

zf U6a promoter
gRNA backbone

Use

CRISPR

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-NDP52

Plasmid

#175749

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2

Depositor

Wade Harper

Article

Heo et al Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10

Insert

NDP52/CALCOCO2 (CALCOCO2 Human)

Use

Lentiviral

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-TIMER

Plasmid

#103056

Purpose

medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaRING

Plasmid

#124952

Purpose

Mammalian expression of Human TRIM28 with deleted RING domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of RING domain

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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VP64-dCas9

Plasmid

#177171

Purpose

VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

VP64-dCas9(D10A, H840A)

Use

CRISPR

Tags

NLS and NLS-3xFLag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Article

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA11-mRuby3

Plasmid

#184894

Purpose

Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA11 (ANXA11 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R230C relative to NP_001148.1

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGL-p21UTR

Plasmid

#20014

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR (Cdkn1a Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
XE118 CAMKII-T286D-CS2+

Plasmid

#16736

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-T286D (Camk2a Rat)

Use

Xenopus expression

Mutation

threonine 286 mutated to aspartate

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pTrEno

Plasmid

#115474

Purpose

Eno-promoter driven expression vector for protein production in Trichoderma reesei

Depositor

Steve Decker

Article

Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

Insert

Cel7A

Use

Unspecified

Mutation

Introns have been removed

Promoter

Trichoderma reesei Enolase (Eno) promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHsp18.2 (GB0153)

Plasmid

#68171

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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