We narrowed to 28,416 results for: REP

1. pCENPBR pc861

   Plasmid

   #167569

   Purpose

   Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   Homo sapiens centromere protein B (CENPB Human)
   

   Use

   Tags

   Ds-Red

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMeCP2miRFP670 pc3942

   Plasmid

   #167568

   Purpose

   The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   MeCp2 (Mecp2 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

   Plasmid

   #136884

   Purpose

   Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   engrailed repressor domain (en Fly)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetO-FUW-MCS-EnR_C-term

   Plasmid

   #136885

   Purpose

   Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   engrailed repressor domain (en Fly)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. kiCAP-AAV-BI-hTFR1

   Plasmid

   #218796

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-TetOn-mCherry-OAZ1_FS-YFP

   Plasmid

   #232356

   Purpose

   Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

   Depositor

   Ankur Jain

   Article

   Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

   Insert

   OAZ1 derived polyamine sensing module (OAZ1 Human)
   

   Use

   Lentiviral

   Tags

   eYFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-Hygro-mTagBFP2

    Plasmid

    #99374

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. kiCAP-AAV-BI-hTFR1-2

    Plasmid

    #218797

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    AAV Rep-Cap BI-hTFR1-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetO-FUW-NfiA

    Plasmid

    #64901

    Purpose

    Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    Nfia (Nfia Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMH-SFB-BRCA2

    Plasmid

    #99395

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

    Depositor

    Michael Huen, Junjie Chen

    Article

    Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)_CBh-Cas9-T2A-BFP

    Plasmid

    #64323

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC476 - pAAV c-fos eYFP

    Plasmid

    #47907

    Purpose

    An AAV vector that expresses eYFP under the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    c-fos

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid

    #218508

    Purpose

    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    mNeongreen
    

    Use

    Tags

    modified ssrA degradation tag

    Expression

    Bacterial

    Mutation

    deletion of MCS containing Plac_lacZa, introducti…

    Promoter

    P23

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 iCasper T2A HO1

    Plasmid

    #64278

    Purpose

    Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMAL-XPG

    Plasmid

    #39215

    Depositor

    Aziz Sancar

    Article

    Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

    Insert

    XPG (ERCC5 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Note that XPG starts at amino acid 5

    Promoter

    Ptac

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. lentiGuide-Hygro-iRFP670

    Plasmid

    #99377

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3 HA-PHLPP1 full length

    Plasmid

    #37100

    Purpose

    Expresses HA tagged, full-length human PHLPP1 in mammalian cells

    Depositor

    Alexandra Newton

    Article

    Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

    Insert

    PHLPP1 (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOTTC590 - pCMV ER-iRFP

    Plasmid

    #59136

    Purpose

    A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    ER-localized Infrared Fluorescent Protein
    

    Use

    Tags

    ER retention signal and signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid

    #160458

    Purpose

    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Inserts

    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    

    Use

    Insertion of a fluorescent reporter through homol…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mARGAna (cassette 1, transient)

    Plasmid

    #197588

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCXLE-hUL + mTAGBFP2

    Plasmid

    #74944

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    LMyc/Lin28 (MYCL, LIN28A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

    Plasmid

    #165445

    Purpose

    Expresses ACX, rPB(8kD) in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rPB(8kD)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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