We narrowed to 7,653 results for: CCH

1. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193315

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Tags

   4FLAG

   Expression

   Bacterial

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN mEGFP

   Plasmid

   #188643

   Purpose

   This is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genes

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRFP720-tubulin

   Plasmid

   #136574

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720-tubulin
   

   Tags

   miRFP720

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVB3011

   Plasmid

   #113389

   Purpose

   Galactose-inducible L-BC gag: cure killer

   Depositor

   Suzanne Sandmeyer

   Article

   Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

   Insert

   L-BC Gag
   

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_Blasto

   Plasmid

   #167856

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPM1003

   Plasmid

   #167550

   Purpose

   Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

   Depositor

   Renato Morona

   Article

   Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

   Insert

   Vibrio cholerae O antigens genes of the Ogawa serotype
   

   Use

   Cosmid

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a (+)-Gal4-vp16

   Plasmid

   #171075

   Purpose

   Expresses Gal4-vp16 fusion protein in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4 DBD (NEWENTRY Budding Yeast)
   

   Tags

   His and VP16 AD (413-490)

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

   Plasmid

   #154817

   Purpose

   Inducible expression of AsiSI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AsiSI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-OPTN

   Plasmid

   #171424

   Purpose

   For the purifcation of OPTN protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   OPTN (OPTN Human)
   

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBPGAL4.2::p65Uw

    Plasmid

    #26229

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4::p65 (RELA Human, Budding Yeast)
    

    Expression

    Insect

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIE2-DBD

    Plasmid

    #21602

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    GAL4 DBD (GAL4 Budding Yeast)
    

    Use

    Gateway destination vector

    Tags

    3xHA and Gateway destination cassette

    Expression

    Insect

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHACK-GAL4>QF2(newHA1)

    Plasmid

    #104873

    Purpose

    HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    T2A-QF2
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTW073

    Plasmid

    #64754

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPMVAu8

    Plasmid

    #98297

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFW2000

    Plasmid

    #224211

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ESCRT-I

    Plasmid

    #17634

    Depositor

    James Hurley

    Article

    Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

    Insert

    Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM6-At4CL-CmCHS-MsCHI

    Plasmid

    #73394

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    MsCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEx-insertion-erm

    Plasmid

    #172210

    Purpose

    For genetic insertion in erythromycin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Genetic insertion in prevotella copri

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEx-insertion-erm-T1T2

    Plasmid

    #172212

    Purpose

    For genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminator

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Genetic insertion in prevotella copri

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB700 lenti gRNA mcherry

    Plasmid

    #167910

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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