We narrowed to 7,926 results for: AMPH

1. pRD403

   Plasmid

   #163108

   Purpose

   Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   mScarlet-I_H-NSdbd
   

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRD128

   Plasmid

   #163097

   Purpose

   Expression of mTurquoise2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   mTurquoise2_H-NSdbd
   

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRD129

   Plasmid

   #163098

   Purpose

   Expression of mEos3.2_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   mEos3.2_H-NSdbd
   

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRD198

   Plasmid

   #163101

   Purpose

   Expression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   mEGFP_H-NSdbd
   

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRD396

   Plasmid

   #163103

   Purpose

   Expression of eYFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   eYFP_H-NSdbd
   

   Expression

   Bacterial

   Promoter

   araBAD promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCM’ CbbQ-

   Plasmid

   #162711

   Purpose

   Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Expression

   Bacterial

   Mutation

   Inactivation of CbbQ subunit of CbbOQ rubisco act…

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCM’ ΔacRAF

   Plasmid

   #162710

   Purpose

   Second CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAF

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Expression

   Bacterial

   Mutation

   pCCM' with a deletion of putative rubisco ch…

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUPD2 TNos:Luc:5'UTR (GB1499)

   Plasmid

   #160580

   Purpose

   Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   Luc
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD2 PhiC31 integrase (GB1561)

   Plasmid

   #160586

   Purpose

   Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUPD2 linker-RDF (GB2892)

    Plasmid

    #160604

    Purpose

    RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR357.12

    Plasmid

    #125107

    Purpose

    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    A84T - see depositor comments below and Q183K-see…

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti C-MYC-DDK Puro PNRC1

    Plasmid

    #123300

    Purpose

    Mammalian expression of PNRC1 C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJOG252

    Plasmid

    #80584

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJOG253

    Plasmid

    #80585

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZM3[I:FruR-L,I:RbsR-L]

    Plasmid

    #60777

    Purpose

    Contains PI driving expression FruR-L, and PI driving expression of RbsR-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    FruR-L
    RbsR-L
    

    Mutation

    LacI/GalR repressor chimera. LacI DBD with FruR L…

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTSlb-R756S

    Plasmid

    #60730

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTSara-R756S

    Plasmid

    #60725

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTSara-Q758C

    Plasmid

    #60724

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ175

    Plasmid

    #29493

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pJ158

    Plasmid

    #29492

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pJ177

    Plasmid

    #29494

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pJ178

    Plasmid

    #29495

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pJ139

    Plasmid

    #29489

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pJ143

    Plasmid

    #29490

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pJ138

    Plasmid

    #29488

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMDC32B-NbmiR482aTS-B/c

    Plasmid

    #227967

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Expression

    Plant

    Promoter

    35S and None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBZCas13b

    Plasmid

    #89898

    Purpose

    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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