9,006 results

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EF-hGPC3

Plasmid

#24670

Depositor

Jorge Filmus

Article

Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

Insert

GPC3 (GPC3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
pETM6-G6-vioABE-4A6-vioC-3A2-vioD

Plasmid

#73440

Purpose

Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC-P3A2-vioD

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTgenomic

Plasmid

#1778

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSNK1D

Plasmid

#23796

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1D (CSNK1D Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
HA-14-3-3 eta

Plasmid

#116887

Purpose

Expresses HA-tagged human 14-3-3 eta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 eta (YWHAH Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 delta N

Plasmid

#17649

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Mutation

Missing 328 N-terminal amino acids

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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Tol2-mpeg1-dendra2

Plasmid

#51462

Purpose

expresses photoconvertible dendra in macrophages

Depositor

Anna Huttenlocher

Article

Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

Insert

mpeg-dendra

Expression

Bacterial

Promoter

mpeg1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-HTNC

Plasmid

#13763

Purpose

For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

Depositor

Klaus Rajewsky

Article

Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

Insert

His-TAT-NLS-Cre

Use

Baculovirus

Tags

His Tag, NLS, and TAT

Expression

Bacterial

Promoter

p10 promoter

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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2C-3XtbGFP-PEST

Plasmid

#69072

Purpose

Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

turboGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-WT

Plasmid

#8984

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[Chronos-GFP]

Plasmid

#62722

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Has Service

AAV6 and AAV8

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2

Plasmid

#11048

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 (CDC73 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-N E4orf1

Plasmid

#38063

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

E4 orf1

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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SIRT7 Flag

Plasmid

#13818

Purpose

Mammalian expression of human SIRT7 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT7 (SIRT7 Human)

Tags

flag

Expression

Mammalian

Mutation

Missing exon 2

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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RelA cFlag pcDNA3

Plasmid

#20012

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

RelA (Rela Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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CIBN-CAAX

Plasmid

#79574

Purpose

expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

CIBN (CIB1 Mustard Weed)

Tags

C-terminal CAAX-box

Expression

Mammalian

Mutation

K93A, R94A, K106A, K107A (please see depositor co…

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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