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pEGFPN3-Sstr3

Plasmid

#35623

Depositor

Kirk Mykytyn

Article

Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

Insert

Sstr3 (Sstr3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-T17N

Plasmid

#12982

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 DN (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

R175H

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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ampkar

Plasmid

#35097

Purpose

A fluorescent reporter of AMPK activity and cellular energy stress

Depositor

Lewis Cantley

Article

Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

Insert

ampkar

Tags

ECFP and Venus

Expression

Bacterial

Mutation

na

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1

Plasmid

#10830

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 (ARF1 Bovine)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pT040

Plasmid

#67640

Purpose

URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Insert

single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α wt-pBabe-Puro

Plasmid

#26055

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14

Plasmid

#46907

Purpose

expresses EGFP tagged Tau E14 in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SNAP29

Plasmid

#45915

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-rab11 DN

Plasmid

#12678

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R249S

Plasmid

#22935

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R249S Mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pLNCX2-Scrib

Plasmid

#24600

Depositor

Senthil Muthuswamy

Article

Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

Insert

Scribble (SCRIB Human)

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla

Plasmid

#60510

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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mRFP-Ub

Plasmid

#11935

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin (RBX1 Human)

Tags

mRFP

Expression

Mammalian

Available Since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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