Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,233 results for: puc

Clear keywords
Showing: 5161 - 5180 of 5233 results

  1. AA324

    Plasmid
    #215953
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215953-plasmid-map-sequence-id-431481

    Enlarge

  2. qTAG-N-Blast-moxGFP-LMNB1

    Plasmid
    #207772
    Purpose
    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Feb. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    207772-plasmid-map-sequence-id-415638

    Enlarge

  3. qTAG-N-Blast-miniTurbo-LMNB1

    Plasmid
    #207774
    Purpose
    Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207774-plasmid-map-sequence-id-416197

    Enlarge

  5. pTS1043

    Plasmid
    #169621
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169621-plasmid-map-sequence-id-366769

    Enlarge

  6. pTS1042

    Plasmid
    #169620
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169620-plasmid-map-sequence-id-365622

    Enlarge

  7. pVEZF1-donor

    Plasmid
    #112341
    Purpose
    CRISPR donor plasmid to tag human transcription factor VEZF1 with GFP
    Depositor
    Kevin White
    Insert
    VEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
    Use
    CRISPR
    Mutation
    rs999907002, hg38:17:57975308:A>T, hg38:17:579…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112341-plasmid-map-sequence-id-229654

    Enlarge

  8. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

    Enlarge

  9. qTAG-C-Solo-mScarlet-H3C2

    Plasmid
    #207781
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207781-plasmid-map-sequence-id-416187

    Enlarge

  10. kiCAP-AAV-PAL1D

    Plasmid
    #196686
    Purpose
    Rep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PTQGTFR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196686-plasmid-map-sequence-id-388011

    Enlarge

  11. kiCAP-AAV-M.Fas.1

    Plasmid
    #196687
    Purpose
    Rep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    TDALTTK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196687-plasmid-map-sequence-id-388008

    Enlarge

  12. kiCAP-AAV-PAL1B

    Plasmid
    #196684
    Purpose
    Rep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PSQGTLR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196684-plasmid-map-sequence-id-389747

    Enlarge

  13. kiCAP-AAV-PAL1A

    Plasmid
    #196683
    Purpose
    Rep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PTQGTVR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196683-plasmid-map-sequence-id-389740

    Enlarge

  14. kiCAP-AAV-M.Fas.4

    Plasmid
    #196690
    Purpose
    Rep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    VVSDYTV insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196690-plasmid-map-sequence-id-389719

    Enlarge

  15. kiCAP-AAV-M.Fas.3

    Plasmid
    #196689
    Purpose
    Rep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    RVDPSGL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196689-plasmid-map-sequence-id-389717

    Enlarge

  16. kiCAP-AAV-M.Fas.2

    Plasmid
    #196688
    Purpose
    Rep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    STIPTMK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    196688-plasmid-map-sequence-id-386840

    Enlarge

  17. pTS2326

    Plasmid
    #169628
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    Sept. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    169628-plasmid-map-sequence-id-372400

    Enlarge

  18. pTS2321

    Plasmid
    #169626
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    Sept. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    169626-plasmid-map-sequence-id-372399

    Enlarge

Showing: 5161 - 5180 of 5233 results