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We narrowed to 16,745 results for: EMB

Showing: 1001 - 1040 of 16745 results

  6. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid
    #114135
    Purpose
    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114135-plasmid-map-sequence-id-224262

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  7. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114138-plasmid-map-sequence-id-215467

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  8. pCDNA3_PKA-Cα(S11A)-mPAGFP

    Plasmid
    #114134
    Purpose
    Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114134-plasmid-map-sequence-id-224258

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  9. pCDNA3_PKA-CαN-mPAGFP

    Plasmid
    #114114
    Purpose
    Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114114-plasmid-map-sequence-id-224230

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  23. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  26. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

    Plasmid
    #69833
    Purpose
    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyTag-C1C2
    Use
    Lentiviral
    Tags
    Trafficking signal and mCherry
    Expression
    Mammalian
    Mutation
    Inserted SpyTag after the signal peptide of C1C2.
    Promoter
    CamKIIa
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69833-plasmid-map-sequence-id-183098

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  28. CD8a-hnRNP-M-EGFP

    Plasmid
    #86054
    Purpose
    A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane domians along with…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86054-plasmid-map-sequence-id-177920

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  30. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  31. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  36. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

    Plasmid
    #224512
    Purpose
    A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    3xHA-BirA-T2A-mCherry-Caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    224512-plasmid-map-sequence-id-446939

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  39. pS238D1::sptse5-CT_phoA-lacZalpha

    Plasmid
    #192961
    Purpose
    Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT_phoA-lacZ_
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1317) fused to phoA-lacZal…
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192961-plasmid-map-sequence-id-380611

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Showing: 1001 - 1040 of 16745 results