We narrowed to 10,921 results for: NSI;

1. VinculinTS-I997A

   Plasmid

   #111828

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed isoleucine 997 to alanine

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-N1-ro1GFP

   Plasmid

   #82369

   Purpose

   Expression of redox sensitive GFP in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S/F64L/T65S/S147C/Q204C

   Promoter

   CMV

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. myr-palm_mCherry2-mEGFP

   Plasmid

   #127816

   Purpose

   Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   myristoylated-palmitoylated-mCherry2-mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET26b_Nb.AT110.i1

   Plasmid

   #135481

   Purpose

   E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110.i1
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS267

   Plasmid

   #122380

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRF0G-Tyr

   Plasmid

   #113644

   Purpose

   Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   MjYRS and tRNA gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. myr-palm_2xmEYFP

   Plasmid

   #127811

   Purpose

   Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   myristoylated-palmitoylated-mEYFP-mEYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1-ro2GFP

    Plasmid

    #82370

    Purpose

    Expression of redox sensitive GFP in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-Chimeric_Butterfly_CY_1.0

    Plasmid

    #59800

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Thy1.1-muSOX

    Plasmid

    #131702

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSETB-ro2GFP

    Plasmid

    #82366

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/S65T/Q80R/S147C/Q204C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUE1-TSP-UL32

    Plasmid

    #162658

    Purpose

    Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-PA(WT)-Vav2

    Plasmid

    #86977

    Purpose

    PI(DM): Photo-activatable/light sensitive

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pADtet-His6-MscL

    Plasmid

    #83373

    Purpose

    Expresses wild type MscL in mammalian cells

    Depositor

    Allen Liu

    Article

    Heureaux et al Cell Mol Bioeng. 2014 Sep;7(3):307-319.

    Insert

    Mechanosensitive channel of large conductance (mscL E. coli)
    

    Use

    Adenoviral

    Tags

    His6 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

17. myr-palm_mCherry-mEGFP

    Plasmid

    #127815

    Purpose

    Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-muSOX

    Plasmid

    #131701

    Purpose

    expresses muSOX with GFP N-terminally fused

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    GFP-muSOX (GAMMAHV.ORF37 MHV68)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-iRFP

    Plasmid

    #64887

    Purpose

    Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

    Depositor

    Tomomi Ichinose

    Article

    Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

    Insert

    iRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. K8.1 Pr pGL4.16

    Plasmid

    #120377

    Purpose

    Firefly luciferase reporter with KSHV K8.1 late gene promoter

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    K8.1 Promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    K8.1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-Chimeric_Butterfly_YR_1.0

    Plasmid

    #59801

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSETB-ro1GFP

    Plasmid

    #82365

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSETB-ro4GFP

    Plasmid

    #82368

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro4GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/S65T/Q80R/N149C/S202C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-PesaR-Clux

    Plasmid

    #47658

    Purpose

    PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-C
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Additional esa box between -35 site and the origi…

    Promoter

    PesaR-C

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA4/TO-2xStrep-BFLF1

    Plasmid

    #162626

    Purpose

    Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. UL79-3xFLAG pCDNA4

    Plasmid

    #120374

    Purpose

    Expresses HCMV UL79 with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    UL79 (UL79 Human Cytomegalovirus (HHV-5))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-Xl-VSP1

    Plasmid

    #51880

    Purpose

    expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AtFLS2pro::GUS

    Plasmid

    #87167

    Purpose

    expression pattern of AtFLS2

    Depositor

    Silke Robatzek

    Article

    Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

    Insert

    AT5G46330 promoter
    

    Use

    Tags

    GUS

    Expression

    Mutation

    Promoter

    AtFLS2

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGlab592

    Plasmid

    #236554

    Purpose

    240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

    Depositor

    Britt Glaunsinger

    Article

    Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

    Insert

    KSHV ORF68 upstream 240bp
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac WT SOX

    Plasmid

    #192055

    Purpose

    Expression vector for WT SOX

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    WT SOX (ORF37)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAMC070n1a12

    Plasmid

    #209746

    Purpose

    ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

    Depositor

    Ahmed Badran

    Article

    Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

    Insert

    tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJMC-5-Esp3I

    Plasmid

    #197836

    Purpose

    Level 1 module 5 with Esp3I expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJMC-3-Esp3I

    Plasmid

    #197832

    Purpose

    Level 1 module 3 with Esp3I expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJMC-3-BsaI

    Plasmid

    #197820

    Purpose

    Level 1 module 3 with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJMC-5-BsaI

    Plasmid

    #197824

    Purpose

    Level 1 module 5 with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJMC-1-BsaI

    Plasmid

    #197816

    Purpose

    Level 1 module 1 with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJMC-2T-BsaI

    Plasmid

    #197817

    Purpose

    Level 1 module 2T with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJMC-2-BsaI

    Plasmid

    #197818

    Purpose

    Level 1 module 2 with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJMC-3T-BsaI

    Plasmid

    #197819

    Purpose

    Level 1 module 3T with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJMC-4T-BsaI

    Plasmid

    #197821

    Purpose

    Level 1 module 4T with BsaI expansion point

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge