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Showing: 1251 - 1300 of 6170 results

  1. pSTAP-seq_fly-zfh1_spikeIn-4

    Plasmid
    #86390
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
    Use
    Stap-seq spike in vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86390-plasmid-map-sequence-id-173960

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  2. pDD284

    Plasmid
    #66825
    Purpose
    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pNM3746

    Plasmid
    #154109
    Purpose
    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
    Depositor
    Michael Nonet
    Article
    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.
    Inserts
    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    Use
    Cre/Lox; Rmce
    Tags
    Expression
    Worm
    Mutation
    Promoter
    not applicable
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154109-plasmid-map-sequence-id-306068

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  9. pQlox66R

    Plasmid
    #135657
    Purpose
    Clostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135657-plasmid-map-sequence-id-305684

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  10. pQadd1R

    Plasmid
    #135661
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135661-plasmid-map-sequence-id-305685

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  11. pQlox71R

    Plasmid
    #135656
    Purpose
    Clostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135656-plasmid-map-sequence-id-305681

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  12. pQlox71F

    Plasmid
    #135655
    Purpose
    Clostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135655-plasmid-map-sequence-id-305679

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  13. pQlox66F

    Plasmid
    #135658
    Purpose
    Clostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135658-plasmid-map-sequence-id-275772

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  16. pLX_305_Ovalbumin

    Plasmid
    #184924
    Purpose
    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.
    Depositor
    Arlene Sharpe
    Article
    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.
    Insert
    Ovalbumin (OVAL Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Our vector has an A188T mutation that does not im…
    Promoter
    PGK
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    184924-plasmid-map-sequence-id-362665

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  17. pAAV-TetON-CD4

    Plasmid
    #217537
    Purpose
    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    CD4 receptor (CD4 Human)
    Use
    AAV
    Tags
    HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    TetOperon (TetO)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217537-plasmid-map-sequence-id-429499

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  18. pAG416GAL-1x-ccdB-6stop

    Plasmid
    #203519
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203519-plasmid-map-sequence-id-402864

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  19. pNBU2_repA1

    Plasmid
    #211910
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211910-plasmid-map-sequence-id-421620

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  20. pNBU2_repA3

    Plasmid
    #211912
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211912-plasmid-map-sequence-id-421619

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  21. pNBU2_repA2

    Plasmid
    #211911
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211911-plasmid-map-sequence-id-421618

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  22. pSPAsp-hp

    Plasmid
    #48122
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    predicted optimal signal peptide for Bacillus megaterium protein secretion
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48122-plasmid-map-sequence-id-69949

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  23. iChr2-Notch Mosaic (SO250)

    Plasmid
    #99752
    Purpose
    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes
    Depositor
    Rui Benedito
    Article
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99752-plasmid-map-sequence-id-182454

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  24. Lenti-LucAL

    Plasmid
    #174049
    Purpose
    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucAL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…
    Promoter
    Human Ubiquitin C
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    174049-plasmid-map-sequence-id-345204

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  25. Lenti-LucA

    Plasmid
    #174047
    Purpose
    Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucA
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine
    Promoter
    Human Ubiquitin C
    Available since
    Availability
    Academic Institutions and Nonprofits only
    174047-plasmid-map-sequence-id-345203

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  26. Lenti-LucL

    Plasmid
    #174048
    Purpose
    Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    LAMA4 glycine 1254 to valine
    Promoter
    Human Ubiquitin C
    Available since
    Availability
    Academic Institutions and Nonprofits only
    174048-plasmid-map-sequence-id-345139

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  36. MZB129

    Plasmid
    #228483
    Purpose
    "Empty" HSA-secretion vector for easy DNA Assembly (Mammalian cell Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228483-plasmid-map-sequence-id-455748

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  37. pAG416GAL-3x-uC-ccdB-6stop

    Plasmid
    #203514
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203514-plasmid-map-sequence-id-402866

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  38. pAG416GAL-2x-uA-ccdB-6stop

    Plasmid
    #203517
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203517-plasmid-map-sequence-id-402863

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  40. MZB126

    Plasmid
    #228482
    Purpose
    "Empty" HA-secretion (membrane) vector for easy DNA Assembly (Mammalian cell Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228482-plasmid-map-sequence-id-455286

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  41. ORFtag_LambdaN-SD_Frame3

    Plasmid
    #220986
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220986-plasmid-map-sequence-id-437120

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  42. ORFtag_LambdaN-SD_Frame1

    Plasmid
    #220984
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220984-plasmid-map-sequence-id-437511

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  43. ORFtag_LambdaN-SD_Frame2

    Plasmid
    #220985
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220985-plasmid-map-sequence-id-437496

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  47. pTD-NStrepHis

    Plasmid
    #45936
    Purpose
    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Trc (lactose/IPTG inducible)
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45936-plasmid-map-sequence-id-64475

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  50. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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Showing: 1251 - 1300 of 6170 results