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Showing: 1251 - 1300 of 12528 results

  16. p123shy1-mCherry-HA

    Plasmid
    #67875
    Purpose
    encodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.
    Depositor
    Armin Djamei
    Article
    Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do
    Insert
    shy1
    Use
    Expression in ustilago maydis
    Tags
    HA and mCherry
    Expression
    Mutation
    Promoter
    shy1
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67875-plasmid-map-sequence-id-121040

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  20. pcDNA3-CTE-Luc

    Plasmid
    #60881
    Purpose
    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE
    Depositor
    Vinay Nandicoori
    Article
    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371
    Insert
    Luc-CTE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    60881-plasmid-map-sequence-id-98231

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  32. CSTI.pJL1

    Plasmid
    #199113
    Purpose
    In vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrate
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    CSTI
    Use
    Tags
    CAT linker, Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199113-plasmid-map-sequence-id-390798

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  34. AAVS1-TRE3G-NODALvar-matMYC

    Plasmid
    #115641
    Purpose
    For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115641-plasmid-map-sequence-id-232862

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  35. NODALvar-MYC-DYK-N328A

    Plasmid
    #115246
    Purpose
    For mammalian expression of a human NODAL splice variant open reading frame (with a mutated N-glycosylation site) with a C-terminal MYC-DYK tag
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL splice variant (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N328A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115246-plasmid-map-sequence-id-222166

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  44. pCJR01

    Plasmid
    #187888
    Purpose
    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Insert
    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187888-plasmid-map-sequence-id-368412

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  45. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Use
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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Showing: 1251 - 1300 of 12528 results