We narrowed to 8,828 results for: Pol

1. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

   Plasmid

   #192552

   Purpose

   Expresses nuclear localized tdTomato under CAG promoter

   Depositor

   Guoping Feng

   Has Service

   AAV PHP.eB and AAV9

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   deleted E273 and G274 compared to FPbase ID: PGG5S

   Promoter

   CAG

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-EGFP barcode-1-SV40 polyA

   Plasmid

   #190865

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-EGFP barcode-0-SV40 polyA

   Plasmid

   #190864

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-EGFP barcode-3-SV40 polyA

   Plasmid

   #190867

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-EGFP barcode-4-SV40 polyA

   Plasmid

   #190868

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode4
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-EGFP barcode-2-SV40 polyA

   Plasmid

   #190866

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. KL157 - pBlueScript II SK-Grb10_HA-T2A-eGFP-SV40PolyA

   Plasmid

   #232935

   Purpose

   Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Grb10 locus. Used with PX459-sgRNA048_Grb10 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   T2A-eGFP-SV40pA with Grb10 homology arms
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. single polypeptide FPX biosensor for calcium ion

   Plasmid

   #60887

   Purpose

   FPX biosensor

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for calcium ion
   

   Use

   Tags

   HindIII site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-TgKI-MCS-eGFP-MCS-polyA

    Plasmid

    #174024

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

    Plasmid

    #218868

    Purpose

    Mammalian expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

    Plasmid

    #218869

    Purpose

    Mammalian expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    CAG

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

    Plasmid

    #84604

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-RHO-IntC-dCas9C-VPR-polyA

    Plasmid

    #166692

    Purpose

    Express C-terminal part of split dCas9-VPR

    Depositor

    Stylianos Michalakis

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Insert

    IntC-dCas9C-VPR
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-TgKI-MCS-p2A-tdTomato-polyA

    Plasmid

    #174027

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-tdTomato
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

    Plasmid

    #154004

    Purpose

    Express CasRx

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

    Plasmid

    #193968

    Purpose

    Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    hPol eta (POLH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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