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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,655 results for: Pol

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Showing: 501 - 520 of 7655 results

  5. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Publication
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139836-plasmid-map-sequence-id-308228

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  8. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Publication
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    p10 and polyhedrin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65214-plasmid-map-sequence-id-116341

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  9. C1A-C1A-EYFP

    Plasmid
    #61155
    Purpose
    Diacylglycerol (DAG) reporter
    Depositor
    Tobias Meyer
    Publication
    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    C1A from PKCG amino acids 26-89
    Promoter
    CMV
    Available since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    61155-plasmid-map-sequence-id-99172

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  10. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Publication
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72325-plasmid-map-sequence-id-137259

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  14. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Publication
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    159107-plasmid-map-sequence-id-310025

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  16. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid
    #49333
    Purpose
    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Publication
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CACNA1D (Cacna1d Rat)
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, 31b and 42a.Deletion of ex…
    Promoter
    CMV
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    49333-plasmid-map-sequence-id-159439

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  20. p406TEF1

    Plasmid
    #15976
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

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Showing: 501 - 520 of 7655 results