We narrowed to 4,333 results for: U6 gRNA

1. psgRNA-GST-EGFP-GPIDAF

   Plasmid

   #213719

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psgRNA-GST-EGFP-GPICEAM7

   Plasmid

   #213721

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-U6-AlbEx14-mNG

   Plasmid

   #201780

   Purpose

   knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

   Depositor

   Hajime Hirase

   Article

   Vittani et al Fluorescence Imaging of the Brain. Neuro

   Inserts

   Alb gRNA (Alb Mouse)
   Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM-U6-reRNA-mmDNMT1

   Plasmid

   #205635

   Purpose

   reRNA guide targeting Mus Musculus DNMT1

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-mmDNMT1 (Dnmt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9-sgRNA-A+B

   Plasmid

   #149370

   Purpose

   Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   Cas9
   sgRNA-A
   sgRNA-B
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   3xFLAG, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, PGK, and U6

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #173211

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

   Plasmid

   #173212

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

   Plasmid

   #173213

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pepper-fused sgRNA

    Plasmid

    #217520

    Purpose

    Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    Pepper-fused sgRNA for spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid

    #172841

    Purpose

    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330_TUBA1B sgRNA / hSpCas9

    Plasmid

    #172834

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330_CALR sgRNA / hSpCas9

    Plasmid

    #172838

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKSB-AeU6-3gRNA

    Plasmid

    #183914

    Purpose

    gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    gRNA scaffold for protospacer cloning into BbsI sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti U6 promoter (AAEL017763)

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330_Actb sgRNA / hSpCas9

    Plasmid

    #172832

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid

    #172835

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKSB-AeU6-1gRNA

    Plasmid

    #183912

    Purpose

    gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    gRNA scaffold for protospacer cloning into BbsI sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti U6 promoter (AAEL017702)

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKSB-AeU6-2gRNA

    Plasmid

    #183913

    Purpose

    gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    gRNA scaffold for protospacer cloning into BbsI sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti U6 promoter (AAEL017774)

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge