We narrowed to 13,644 results for: NTS

1. pcDNA3.1 LIC 6A HIS-TEV-DPP8

   Plasmid

   #166793

   Purpose

   Mammalian cell expression vector for Flag-tagged DPP8

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP8 (DPP8 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 LIC 6A FLAG-TEV-DPP8

   Plasmid

   #166795

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP8

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP8 (DPP8 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac HTB DPP9L

   Plasmid

   #166788

   Purpose

   Insect cell expression vector for DPP9L (protein purification)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CD 44 pGL4basic

   Plasmid

   #78135

   Purpose

   luciferase reporter for miRNA activity

   Depositor

   Heidi Schwarzenbach

   Article

   Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

   Insert

   CD 44 (CD44 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CD44

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_GCGR-NTEV-TCS-GV-2xHA

   Plasmid

   #194374

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   GCGR-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac HTB DPP9S

   Plasmid

   #166787

   Purpose

   Insect cell expression vector for DPP9S (protein purification)

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Puro-TDP-43-D169G

   Plasmid

   #133757

   Purpose

   Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed aspartate 169 to glycine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Rs7327-RBD-8h

   Plasmid

   #161823

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCPmacroH2A.1-GS3

    Plasmid

    #204759

    Purpose

    Expression of Cas9 and human macroH2A.1

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    macroH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROF141

    Plasmid

    #155329

    Purpose

    Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINT-PuroDDHNF4a2

    Plasmid

    #31443

    Depositor

    Gerhart Ryffel

    Article

    Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Phic31/attb

    Tags

    FKBP L106P

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KHBD00044

    Plasmid

    #34206

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17117 (hth Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pUC19-E3CY56

    Plasmid

    #103075

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMOD_C2520

    Plasmid

    #91089

    Purpose

    Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU6

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Rs4084-RBD-8h

    Plasmid

    #161825

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Rs4231-RBD-8h

    Plasmid

    #161824

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3_DRD1-NTEV-TCS-GV-2xHA

    Plasmid

    #194358

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    DRD1-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac HTB DPP9S S730A

    Plasmid

    #169946

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    S730A

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

    Plasmid

    #117384

    Purpose

    An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

    Plasmid

    #200634

    Purpose

    Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiPGK Puro DEST JNKKTR AE Clover

    Plasmid

    #90240

    Purpose

    Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (AE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SPOP-V5 (BTB+BACK)

    Plasmid

    #169723

    Purpose

    Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K, Y353E

    Promoter

    CMV

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mH2A2-CT-MYC

    Plasmid

    #45170

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy2 (MACROH2A2, H2AFY2 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pRC/CMV-JAK1P1084A-VSV

    Plasmid

    #139358

    Purpose

    Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    P1084A

    Promoter

    CMV

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FusionRed-Dectin1A-C-10

    Plasmid

    #56111

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Dectin1A (CLEC7A Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. MSCV N-term HA-FLAG puro BRD7

    Plasmid

    #41934

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    HA-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pRR.1

    Plasmid

    #69995

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge