We narrowed to 16,573 results for: por

1. pMRXIP Turquoise2-Syntaxin17

   Plasmid

   #89938

   Purpose

   Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

   Insert

   human Syntaxin 17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Avr2-GFP pZK538

   Plasmid

   #118011

   Purpose

   expresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .

   Depositor

   FLW Takken

   Article

   et al Bio-protocol 8(24): e3114.

   Inserts

   dspAvr2
   mCherry
   

   Use

   Tags

   EGFP and HDEL

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTD-NStrepHis

   Plasmid

   #45936

   Purpose

   use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PEP115E

   Plasmid

   #97401

   Purpose

   binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Golgi target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEP116E

   Plasmid

   #97402

   Purpose

   binary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Plasma membrane target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PKAcs-GGSD5X-GFP

   Plasmid

   #108584

   Purpose

   Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

   Depositor

   Robert Hughes

   Article

   Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

   Insert

   protein kinase A, catalytic subunit
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H87Q and W196R

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pG108-K - AhpC-Glow

   Plasmid

   #185135

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Inserts

   Glow gene
   Bacteroides thetaiotaomicron ahpC promoter
   

   Use

   Pg106

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ShuttleNSE

    Plasmid

    #50958

    Purpose

    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

    Depositor

    James Bamburg

    Article

    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NSE

    Available since

    Feb. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. mKO2-C1

    Plasmid

    #54494

    Purpose

    Localization: C1 Cloning Vector, Excitation: 551, Emission: 565

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-PHYTipi

    Plasmid

    #53283

    Purpose

    Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-ZnT1-WT

    Plasmid

    #82340

    Purpose

    Expresses FLAG-tagged ZnT1-WT in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    ZnT1-WT (SLC30A1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-MinE-EGFP_IRES-Blast

    Plasmid

    #213113

    Purpose

    Expression of MinE-EGFP in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-hsRPA4 (MSW#517)

    Plasmid

    #208070

    Purpose

    Expression of GFP-tagged hsRPA4 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

    Insert

    RPA4 (RPA4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXTL-T7max-FLuc

    Plasmid

    #188311

    Purpose

    Expression of Firefly luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Use

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    chicken actin

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-eNOS-GFP

    Plasmid

    #22444

    Depositor

    William Sessa

    Article

    Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

    Insert

    WT eNOS-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDG04439

    Plasmid

    #216365

    Purpose

    xhNup133-Nb2t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup133-Nb2t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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