We narrowed to 23,191 results for: his

Showing: 10401 - 10440 of 23191 results

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pET-PLS-KP2_rpoA-U

Plasmid

#190956

Purpose

Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-A66S_rpoA-U

Plasmid

#190969

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:A66S

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3_rpoA-U

Plasmid

#190960

Purpose

Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-E67A_rpoA-U

Plasmid

#190973

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 FYN

Plasmid

#67106

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FYN (FYN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
CKAP2 pHAT

Plasmid

#175946

Purpose

Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Tags

6-His and StrepTagII

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CREBZF

Plasmid

#67070

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CREBZF (CREBZF Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pmCellFree_KA2_ZIKV NS2A

Plasmid

#145363

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

His and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJK2067

Plasmid

#171845

Purpose

C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

Depositor

Judith Kimble

Article

Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

Insert

his-58, eGFP, mCherry

Tags

N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

Expression

Worm

Mutation

boxB wild type and hairpin mutant

Promoter

mex-5 promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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K339CLM G234A/215/347

Plasmid

#24449

Depositor

Ron Vale

Article

Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

Insert

Kinesin (KIF5B Human)

Tags

His

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E…

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Chicken, Synthetic)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 nu

Plasmid

#44940

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 nu (GRF7 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CSNK2A1

Plasmid

#67085

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFS217-USP44_MSCV-TAP

Plasmid

#43896

Purpose

Retroviral expression of USP44 in mammalian cells

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Retroviral

Tags

FLAG, HA, and HIS

Expression

Mammalian

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only
LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Tags

His

Expression

Bacterial

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2A1

Plasmid

#67076

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2A1 (GTF2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
K347CLM G234A/43/347

Plasmid

#24454

Depositor

Ron Vale

Article

Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

Insert

Kinesin (KIF5B Human)

Tags

His

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BTF3

Plasmid

#67059

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BTF3 (BTF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA6-rbN1 S77A

Plasmid

#32945

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Change Serine77 to Alanine

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFR092

Plasmid

#134148

Purpose

Expression of CENP-T

Depositor

Iain Cheeseman

Article

Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

Insert

CENP-T (CENPT Human)

Tags

His

Expression

Bacterial

Mutation

aa 1-375

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 TYK2

Plasmid

#67133

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TYK2 (TYK2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

A435S

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2H4

Plasmid

#67094

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H4 (GTF2H4 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA6-rbN1 S77D

Plasmid

#32947

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

slc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Serine 77 mutated to Aspartic Acid

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-N1-PDZ1-GAGA

Plasmid

#31651

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His, S-tag

Expression

Mammalian

Mutation

changed P(21)NGYGF changed to H(21)NGAGA

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-N1-PDZ2-GAGA

Plasmid

#32857

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His,S-tag

Expression

Mammalian

Mutation

PDZ2 P(161)NGYGF mutated to H(161)NGAGA

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 MAPK13

Plasmid

#67077

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

MAPK13 (MAPK13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pANY1

Plasmid

#110949

Purpose

Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

Depositor

Yingfeng An

Article

Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

Insert

ccd B cassette

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 ATF3

Plasmid

#67057

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ATF3 (ATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

M2I

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 I136A, I139A, M142A

Plasmid

#74617

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGB1-mCRT(P-domain)

Plasmid

#83519

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Tags

GB1 and His

Expression

Bacterial

Mutation

P domain (contains residues 187-283)

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(C-domain)

Plasmid

#83518

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Tags

GB1 and His

Expression

Bacterial

Mutation

C domain (contains residues 340-399)

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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