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p357_DG

Plasmid

#234700

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p356_DG

Plasmid

#234699

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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InterCatch-3xL

Plasmid

#186909

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->HA-mtC11L34

Plasmid

#162227

Purpose

Lentiviral vector for the expression of HA mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mtC11L34

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET his-mRFP

Plasmid

#113553

Purpose

For bacterial expression and purification of mRFP

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mRFP

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1018

Plasmid

#108975

Purpose

shuttle vector, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1021

Plasmid

#108978

Purpose

shuttle vector, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_C3013

Plasmid

#91100

Purpose

Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0201

Plasmid

#91002

Purpose

Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0202

Plasmid

#91003

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

AtUbi10

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-rpiL*

Plasmid

#90320

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS rpiL*

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV651

Plasmid

#87631

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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Rb379-928/C706F-pGex2TK

Plasmid

#21750

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928 of wt Rb …

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA

Plasmid

#220893

Purpose

Express haA3A-CBE-VA (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G-NG

Plasmid

#220897

Purpose

Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A-NG

Plasmid

#220895

Purpose

Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G

Plasmid

#220894

Purpose

Express haA3A-CBE-G (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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