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pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD1_1

Plasmid

#36365

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD1_2

Plasmid

#36366

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pZA27

Plasmid

#158505

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc (ZAR1 Mustard Weed)

Tags

4xMyc

Expression

Plant

Mutation

L17E

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD2_3

Plasmid

#36370

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD1_3

Plasmid

#36367

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-NR2F1_2

Plasmid

#36363

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pRSV-p65T305A,S276A

Plasmid

#106456

Purpose

NF-kB p65, kinase sites 276,305 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase sites 276,305 mutated (RELA Human)

Expression

Mammalian

Mutation

kinase sites inactivated

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-NR2F1_3

Plasmid

#36364

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-NR2F1_1

Plasmid

#36362

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
ABEx2_WT_GFP (ABE8e_L34W_nCas9_WT)

Plasmid

#251549

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY)

Plasmid

#251545

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT)

Plasmid

#251551

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_SpRY (ABE8e_V28C_M151E_nCas9_SpRY)

Plasmid

#251547

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_WT_GFP (ABE8e_V28C_nCas9_WT)

Plasmid

#251548

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY)

Plasmid

#251546

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY_V106W (ABE8e_V28C_nCas9_SpRY_V106W)

Plasmid

#251552

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY_V106W adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY_V106W

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY (ABE8e_V28C_nCas9_SpRY)

Plasmid

#251544

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge