We narrowed to 29,593 results for: REP

1. pLV-Hygro-EF1A-EGFP-PARP1

   Plasmid

   #176146

   Purpose

   EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherryC1 VAP-A KD/MD

   Plasmid

   #226408

   Purpose

   Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   VAP-A (VAPA Human)
   

   Use

   Tags

   T7-Xpress tag and mCherry

   Expression

   Mammalian

   Mutation

   K94D/M96D mutant, unable to bind FFAT motifs

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

   Plasmid

   #51248

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907,980,1006 mutated to A…

   Promoter

   EF1a

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pF CAG luc puro

   Plasmid

   #67501

   Purpose

   Constitutive expression of firefly luciferase in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-102.1F10-IgG1/λ

   Plasmid

   #50366

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG1/λ antibody isotype (102.1F10-IgG1/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 1 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213973

   Purpose

   AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGG-B1

   Plasmid

   #78504

   Purpose

   Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV-B1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-human HoxB4-IRES-YFP

   Plasmid

   #91889

   Purpose

   Retroviral expression of human HoxB4

   Depositor

   Martine Roussel, Brian Sorrentino

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   HoxB4 (HOXB4 Human)
   

   Use

   Retroviral

   Tags

   IRES-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV gag

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Sox2-CreERT2

   Plasmid

   #135618

   Purpose

   Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   P2A-CreERT2

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TBK1-C-FLAG-HA

    Plasmid

    #131792

    Purpose

    Lentiviral vector expressing WT TBK1 containing STOP codon

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    STOP codon

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN441

    Plasmid

    #137873

    Purpose

    PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Parvalbumin scFv [L114/3]

    Plasmid

    #190504

    Purpose

    Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
    

    Use

    Tags

    HA, 6xHis, Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tom20-CR

    Plasmid

    #171461

    Purpose

    Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX_TRC311 ETV4-L

    Plasmid

    #74982

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S396N

    Promoter

    EF1a

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-hAPOE-R-loop

    Plasmid

    #134901

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    APOE promoter (APOE Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBbleo-EGF-ScNeo

    Plasmid

    #209902

    Purpose

    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EGF-ScNeo (EGF )
    

    Use

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXN2-HA-AT1R-YFP

    Plasmid

    #101659

    Purpose

    Expresses AT1R with HA tag and YFP in mammalian cells.

    Depositor

    Yusuke Ohba

    Article

    Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

    Insert

    Angiotensin II type 1 receptor (AGTR1 Human)
    

    Use

    Tags

    HA and YFP (Venus)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. VGlut2 scFv [N29/29]

    Plasmid

    #182085

    Purpose

    Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSIIpuro-AREG-ScNeo

    Plasmid

    #209897

    Purpose

    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    AREG-ScNeo (AREG Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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