Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,278 results for: Vars;

Showing: 7831 - 7860 of 11278 results

  5. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid
    #223114
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223114-plasmid-map-sequence-id-447638

    Enlarge

  10. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179905-plasmid-map-sequence-id-351283

    Enlarge

  12. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

    Enlarge

  17. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

    Enlarge

  22. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

    Enlarge

  24. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

    Enlarge

  27. pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A

    Plasmid
    #82176
    Purpose
    Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection. (Please note this plasmid contains S45A mutation, not T45A)
    Depositor
    William Hahn, David Root, Jesse Boehm
    Article
    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi
    Insert
    CTNNB1 (CTNNB1 Human)
    Use
    Gateway entry vector
    Mutation
    S33A, S37A, T41A, S45A
    Promoter
    None
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82176-plasmid-map-sequence-id-163897

    Enlarge

  28. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid
    #223079
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223079-plasmid-map-sequence-id-447658

    Enlarge

Showing: 7831 - 7860 of 11278 results