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PROM5_Ntprrn_rev_chlo

Plasmid

#163952

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_Ntpsba_chlo

Plasmid

#163953

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pKP932

Plasmid

#167652

Purpose

Express colicinB-His6, M261C

Depositor

Kathleen Postle

Article

Skare et al J Bacteriol. 1989 Aug;171(8):4442-7. doi

Insert

cba-his6 M261C

Expression

Bacterial

Mutation

cba-his6 with M261C mutation

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEG02

Plasmid

#154137

Purpose

This plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

Depositor

Kang Zhou

Article

Panda et al Biochemical Engineering Journal

Insert

fucO, aldA

Expression

Bacterial

Promoter

P_thrC3

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-313 (attR4-attR3)

Plasmid

#161898

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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PAJ408

Plasmid

#104150

Purpose

dCas9 plasmid expressing full length mKate2 mRNA without RBS

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-eCas9-D1135E

Plasmid

#154926

Purpose

aTc inducible eCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

eCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-OptiCas9-D1135E

Plasmid

#154928

Purpose

aTc inducible OptiCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

OptiCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

R661A, K1003H, D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9-D1135E

Plasmid

#154931

Purpose

aTc inducible Cas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0027

Plasmid

#162309

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0549

Plasmid

#162300

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, SUMO-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0550

Plasmid

#162301

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-SUMO-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0539

Plasmid

#162306

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, TEV protease (S219V), no stop codon

Use

Synthetic Biology

Mutation

S219V, codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0551

Plasmid

#162303

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0547

Plasmid

#162298

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0546

Plasmid

#162297

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, MBP-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0544

Plasmid

#162295

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, MBP-[Factor Xa cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0543

Plasmid

#162294

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, GST-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0541

Plasmid

#162292

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, GST-[thrombin cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2373

Plasmid

#160079

Purpose

Z. mobilis CRISPRi, promoter F, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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