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MAC-tag-N

Plasmid

#108078

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Publication

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Promoter

Dox-inducible

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

Plasmid

#100853

Purpose

AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has service

AAV1 and AAV Retrograde

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

1 Data Hub Report

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pH-nCas9-PBE

Plasmid

#98163

Purpose

Expresses CRISPR base editor in rice

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-nCas9-UGI-NLS

Use

Rice

Expression

Plant

Promoter

Ubi

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

Plasmid

#86233

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDMyc-TNPO1

Plasmid

#86081

Purpose

A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Tags

Myc

Expression

Mammalian

Promoter

T7

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1 YFP

Plasmid

#84912

Purpose

Mammalian expresion of TDP-43 NLS1 YFP

Depositor

Aaron Gitler

Publication

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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hKDR

Plasmid

#83436

Purpose

human VEGFR2 with V5 and tGFP tag

Depositor

Christie Thomas

Publication

Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human VEGFR2 (KDR Human)

Tags

V5 and turboGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pIND(V5)EcoDam

Plasmid

#59201

Purpose

Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

Depositor

Bas van Steensel

Publication

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MIG-Luciferase-IRES-GFP

Plasmid

#75021

Purpose

Encodes the expression of Luciferase and GFP

Depositor

Saradhi Mallampati

Insert

Luciferase

Use

Luciferase and Retroviral

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-EFS-PCP-3XGFPnls

Plasmid

#75385

Purpose

PCP-3XGFPnls

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

EFS

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pKanCMV-mRuby3-10aa-H2B

Plasmid

#74258

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to H2B.

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Publication

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

Plasmid

#65418

Purpose

AAV packaging vector for CamKIIa driven KOR DREADD expression

Depositor

Bryan Roth

Publication

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Insert

CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Promoter

CaMKIIa

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA3.1-Flag-H2A

Plasmid

#63560

Purpose

Expression of human H2A tagged with Flag

Depositor

Titia Sixma

Publication

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC13 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAVS1-SA-2A-NEO-CAG-RTTA3

Plasmid

#60431

Purpose

neomycin resistance; constitutive promoter driving reverse tet activator

Depositor

Paul Gadue

Publication

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

rtTA3

Use

Zfn donor

Promoter

CAG

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMito-mCherry-FRB

Plasmid

#59352

Purpose

Expresses mCherry-tagged MitoTrap in mammalian cells.

Depositor

Stephen Royle

Publication

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Tags

FRB and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pAAV-hSyn Con/Fon EYFP

Plasmid

#55650

Purpose

Cre-on/Flp-on EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV8 and AAV Retrograde

Insert

eYFP with introns

Use

AAV; Cre on/flp on eyfp

Expression

Mammalian

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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