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pOTTC475 - pAAV c-fos iRFP

Plasmid

#47906

Purpose

An AAV vector that expresses iRFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

c-fos

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-EGFP

Plasmid

#114213

Purpose

AAV vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRVL-1

Plasmid

#104579

Purpose

Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Expression

Mammalian

Available Since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

Plasmid

#188375

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111590

Purpose

An AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mTurquoise2

Plasmid

#104059

Purpose

AAV expression of Cre-dependent reporter mTurquoise2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mTurquoise2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pVITRO1-102.1F10-IgM/λ

Plasmid

#61880

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hChATp-tdTomato

Plasmid

#197564

Purpose

Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

Depositor

Casey Maguire

Article

Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

Insert

tdTomato

Use

AAV

Promoter

human ChAT promoter

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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011LP-pAAV-TNT4-EmGFP-pA

Plasmid

#120280

Purpose

AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

EmGFP

Use

AAV

Tags

3XFlag and HA

Promoter

TNT Cardiac Troponin T promoter

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2B-GFP

Plasmid

#116869

Purpose

Nuclear localized GFP

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Has Service

AAV Retrograde

Insert

GFP

Use

AAV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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FUGW-d2GFP-200

Plasmid

#79602

Purpose

FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP + miR-200 family targets

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Cas9C-VPR

Plasmid

#80933

Purpose

Expresses Cas9C-VPR in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C-VPR

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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dg265_pENTR_CoChRCel

Plasmid

#66103

Purpose

Entry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

CoChR

Use

Gateway Cloning

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJTX-GL3

Plasmid

#11981

Purpose

Reporter gene with mouse c-jun promoter and point mutation of AP1 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP-mKate2.5f

Plasmid

#99129

Purpose

Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mKate2.5f

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2-GFP

Plasmid

#58881

Purpose

AAV expression of ChR2-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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