We narrowed to 40,900 results for: Eras
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TagBFP(S2h)3(S4h)3-gs-P6f
Plasmid#228083PurposeMammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2h, S4h, P6f
ExpressionMammalianAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R104A
Plasmid#227358PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin R104A (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI3-3×FLAG
Plasmid#215511PurposeExpresses FLAG tagged human WIPI3.DepositorInsertWD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)
UseRetroviralTags3×FLAGExpressionMammalianAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin Ubl P37L
Plasmid#227366PurposeFor bacterial expression of mutated parkin Ubl.DepositorInsertParkin 1-76 P37L (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin D106A
Plasmid#227362PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin D106A (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin V105K
Plasmid#227361PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin V105K (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin T103A
Plasmid#227360PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin T103A (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin L102K
Plasmid#227359PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin L102K (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5419_p3WJdB-glcO36
Plasmid#221193PurposeGlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminatorDepositorInsertT7 promoter-glcO36
UseSynthetic BiologyExpressionBacterialPromoterT7 promoter with downstream glcO36 operatorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF149 shRNA-TRE
Plasmid#225337PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertRNF149 shRNA (Rnf149 Mouse)
UseAAV and RNAiExpressionMammalianPromoterTRE (tetracycline-responsive element)Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 shRNA-TRE
Plasmid#225338PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertUSP7 shRNA (Usp7 Mouse)
UseAAV and RNAiExpressionMammalianPromoterTRE (tetracycline-responsive element)Available SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Q54A
Plasmid#217912PurposeA plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutationDepositorInsertPXP Q54A
Tags6x His (N and C term)ExpressionBacterialMutationGlutamine at position 55 and 267 mutated to Alani…PromoterT5Available SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
T40A
Plasmid#217911PurposeA plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutationDepositorInsertPXP T40A
Tags6x His (N and C term)ExpressionBacterialMutationThreonine at position 41 and 253 mutated to Alani…PromoterT5Available SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-hWIPI2B-3×FLAG
Plasmid#215510PurposeExpresses FLAG tagged human WIPI2B.DepositorInsertWD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
UseRetroviralTags3×FLAGExpressionMammalianAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
Available SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 43-684
Plasmid#205056PurposeTWNK protein expressionDepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 360-684
Plasmid#205055PurposeTWNK protein expressionDepositorAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-GlcisPT
Plasmid#203172PurposeYeast expression vector for Giardia cis-prenyltransferaseDepositorInsertUPPS
ExpressionYeastPromoterTDH3Available SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWalium Htt19Q-mCherry
Plasmid#201248Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertHuntingtin (HTT Human)
ExpressionInsectAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST ɑSynWT-EGFP
Plasmid#201249Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertɑ-Synuclein (SNCA Human)
ExpressionInsectAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CALPAIN-7-mCherry, F98D
Plasmid#180653PurposeLentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.DepositorInsertCALPAIN-7
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GCACCCAUACCUUUACAUUAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-Csy4
Plasmid#161763PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
ExpressionPlantPromoterCmYLCV PromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-tRNA
Plasmid#161762PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
ExpressionPlantPromoterCmYLCV PromoterAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-DmCup_417-1133_T
Plasmid#147589PurposeInsect Expression of DmeIF4Ga_578-650-DmCup_417-1133DepositorInsertDmeIF4Ga_578-650-DmCup_417-1133 (eIF4G1 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I
Plasmid#146577PurposeInsect Expression of DmeIF4GaDepositorInsertDmeIF4Ga (eIF4G1 Fly)
ExpressionInsectMutationone non silent mutation K340R compared to the seq…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-Nterm_H
Plasmid#146493PurposeInsect Expression of DmeIF4Ga-NtermDepositorInsertDmeIF4Ga-Nterm (eIF4G1 Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-AAGAB
Plasmid#198332PurposeExpression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAAGAB (AAGAB Human)
TagsGAL4-DNA binding domain (BD) fragmentExpressionYeastPromoterADH1Available SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENmRFPCNAL2 pc1054
Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::ENO::sNluc2
Plasmid#186770PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only
