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pBABE-2xFLAG-2xSTREP-CCND3-Puro

Plasmid

#172622

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag TSSK1B

Plasmid

#20604

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TSSK1B (TSSK1B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pRK-flag-GFP 1-10 tetra Cys

Plasmid

#78589

Purpose

Expression flag-GFP 1-10 tetra Cys in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 1-10 tetra Cys

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PNKP

Plasmid

#20594

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PNKP (PNKP Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-EglN2 H358A-pBabe-puro

Plasmid

#22705

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated cDNA: point mutation in codon 358 changi…

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CKMT2

Plasmid

#20554

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKMT2 (CKMT2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1318

Plasmid

#78587

Purpose

Expression flag-USP19 494-1318 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1318 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-MTS-FLAG-LOV*

Plasmid

#202208

Purpose

Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24

Use

Lentiviral

Tags

FLAG and LOV*

Promoter

Ef1a

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A K113R-3xFlag_IRES-GFP

Plasmid

#198391

Purpose

RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site mutant (K113R)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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1438 pSG5L Flag HA PTEN G129R

Plasmid

#10775

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

G129R

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ITPK1

Plasmid

#20508

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ITPK1 (ITPK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
p8126 MSCV-P C-FlagHA 16E7 R77S

Plasmid

#163305

Purpose

Expresses HPV16 E7 R77S

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV16 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p7612 MSCV-P C-FlagHA 16E7 E10K

Plasmid

#163303

Purpose

Expresses HPV16 E7 E10K

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 E10K

Use

Retroviral

Tags

FlagHA

Mutation

E10K

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-4SA(S164)

Plasmid

#33083

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S61A, S109A, S127A, S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72616

Purpose

Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CSNK1G2

Plasmid

#20471

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CSNK1G2 (CSNK1G2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pKK-RNAi-nucEGFPmiR-FLAG-TEV

Plasmid

#105807

Purpose

Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

RNAi; Flp-in competent

Tags

FLAG-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106354

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

D4Z4 gRNA-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

FLAG/AVI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only