We narrowed to 6,928 results for: tac

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CLTC G10.4 gRNA

Plasmid

#90635

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLTC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CLTC (Guide Designation G10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pPN461

Plasmid

#137877

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC39

Plasmid

#62333

Purpose

sgRNA + 1x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB3053(gRNA X-2, XI-5, XII-4)

Plasmid

#73295

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

Plasmid

#173214

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA

Use

CRISPR; Prime editing (pe3)

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CDC6 B5.1 gRNA

Plasmid

#90596

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B5.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone3

Plasmid

#162121

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-Myocardin

Plasmid

#100769

Purpose

Lentiviral expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-sh-Myocardin

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAT9753-BEAR-mScarlet-preedited

Plasmid

#162994

Purpose

BEAR control plasmid with split mScarlet and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CDC6 B6.1 gRNA

Plasmid

#90597

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC6 (Guide Designation B6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo U6-SpAi9R-U6-SpDMDR

Plasmid

#78609

Purpose

Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9R-U6-SpDMDR

Use

Unspecified

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
EPHB4-gRNA1/2

Plasmid

#139974

Purpose

gRNA used for knockin EGFP into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EPHB4 gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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H2AFX B4.3 gRNA

Plasmid

#90703

Purpose

3rd generation lentiviral gRNA plasmid targeting human H2AFX

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

H2AFX (Guide Designation B4.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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CSNK1A1 H2.4 gRNA

Plasmid

#90641

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK1A1 (Guide Designation H2.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CSNK1A1 H1.4 gRNA

Plasmid

#90640

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1A1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK1A1 (Guide Designation H1.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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3604 pGEX4T-1 Bad S136A (104-141)

Plasmid

#8801

Depositor

Stanley Korsmeyer

Article

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S136A

Promoter

Ptac

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
ACTL6A C8.3 gRNA

Plasmid

#90521

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACTL6A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ACTL6A (Guide Designation C8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD1_2

Plasmid

#104043

Purpose

Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD1 gRNA (GATAD1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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gh52

Plasmid

#106725

Purpose

expression of gRNA targeting ST3GAL4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST3GAL4 (ST3GAL4 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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