We narrowed to 10,749 results for: AGA

1. pBIFC-flag-hCaspase 2 prodomain-VN173

   Plasmid

   #49262

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 prodomain (CASP2 Human)
   

   Use

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   delete aa 148-435

   Promoter

   CMV

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pTBL680 CHYRON3 integration construct

   Plasmid

   #126448

   Purpose

   To integrate the CHYRON3 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6/3xLacO-CHYRON3 hgRNA
   pCMV-puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6/3xLacO

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Raichu-OsRac1 DN

   Plasmid

   #133263

   Purpose

   Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(T24N) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 T24N

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/myc/ER-αT2ib

   Plasmid

   #110769

   Purpose

   Expresses ER intrabody against mouse and human TLR2

   Depositor

   Thomas Böldicke

   Article

   Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

   Insert

   intrabody against mouse and human TLR2
   

   Use

   Tags

   ER retention signal (SEKDEL), ER signal peptide, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. STARR-seq luciferase INF enhancer vector_ORI_OAS3

   Plasmid

   #99324

   Purpose

   Luciferase validation vector with OAS3 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr12: 94575894 -94578286
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pbG-APRT-J-u32uni-PdTKmCh

   Plasmid

   #107279

   Purpose

   APRT c.407T>C (APRT*J) donor vector with unilateral microhomology

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Inserts

   APRT (APRT Human)
   puro-deltaTK; CAG::mCherry
   

   Use

   Tags

   Expression

   Mutation

   c.402A>T, c.407T>C

   Promoter

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

   Plasmid

   #162098

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to HA-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   HA-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-ProtC

   Plasmid

   #162099

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-StrepTagII-ProtC
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-ProtC

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBiFC-HA-hCaspase 2 CARD-VC155

    Plasmid

    #49259

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    Caspase-2 CARD (CASP2 Human)
    

    Use

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    deleted aa 123-435

    Promoter

    CMV

    Available since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

    Plasmid

    #108833

    Purpose

    Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa28 to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS and GAI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBiFC-flag-hCaspase 2 CARD-VN173

    Plasmid

    #49260

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 CARD (CASP2 Human)
    

    Use

    Tags

    Flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 123-435

    Promoter

    CMV

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid

    #49258

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Use

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBABE-puro-Halo-hMRE11(wt)

    Plasmid

    #208393

    Purpose

    To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H129N

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-puro-Halo-hMRE11(H129N)

    Plasmid

    #208394

    Purpose

    To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H129N

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY036_ATP1A1_G3_Array

    Plasmid

    #86619

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

    Plasmid

    #149278

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCLc-U6-shHMGA2.3-PGK-dTomato

    Plasmid

    #89606

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG416GAL-EMCV_3C

    Plasmid

    #201941

    Purpose

    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    EMCV 3C protease (EMCVgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL5.0 hCortKD-Flcortmut3YD EGFP

    Plasmid

    #187264

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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