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We narrowed to 63,680 results for: cloning

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Showing: 5281 - 5300 of 63680 results

  2. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Publication
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  3. pGTVL2-CRBN-TBD

    Plasmid
    #196369
    Purpose
    protein expression of the GST-tagged Cereblon Thalidomide binding domain
    Depositor
    Stefan Knapp
    Insert
    CRBN (CRBN Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only T319-T425
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196369-plasmid-map-sequence-id-385991

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  7. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  10. CBE-T1.52

    Plasmid
    #193285
    Purpose
    Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CBE-T1.52
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193285-plasmid-map-sequence-id-379826

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  13. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  20. p5155

    Plasmid
    #175391
    Purpose
    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Michael Wiles
    Publication
    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    175391-plasmid-map-sequence-id-348372

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Showing: 5281 - 5300 of 63680 results