We narrowed to 59,446 results for: cloning

1. 3xDBE_RE:NLuc

   Plasmid

   #178318

   Purpose

   FOXO NLuc luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   FOXO NLuc Reporter
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS6

   Plasmid

   #55752

   Purpose

   Produces T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   T7 RNA Polymerase
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. NM1-5S-tRNA-SgH

   Plasmid

   #128178

   Purpose

   Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   5S rRNA-tRNA(Arg)

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAGFP-C1

   Plasmid

   #11910

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   PAGFP
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5E-mnx1

   Plasmid

   #82402

   Purpose

   Gateway-compatible zebrafish mnx1 promoter

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   mnx1 promoter
   

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJC53.2

   Plasmid

   #26536

   Purpose

   T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

   Depositor

   Phillip Newmark

   Article

   Collins et al PLoS Biol. 2010 . 8(10):e1000509.

   Type

   Empty backbone

   Use

   T/a cloning vector for dsrna generation and the g…

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTB3-BC2 nanobody

   Plasmid

   #210208

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   BC2 Nanobody
   

   Expression

   Bacterial

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINM

   Plasmid

   #26044

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His-MBP-3C-lacZ
   

   Tags

   MAHHHHHHSSG_MBP_LEVLFQGP

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINE

   Plasmid

   #26043

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His-lacZ-KHHHHHH
   

   Tags

   KHHHHHH and MAHHHHHHSSG

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xHRE_RE:RedF

    Plasmid

    #178314

    Purpose

    HRE RedFirefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    HRE RedFirefly reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL198

    Plasmid

    #180472

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    ZmCas9

    Expression

    Plant

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPRT::DHFR-D

    Plasmid

    #58528

    Purpose

    replace TgUPRT with DHFR by homologous recombination

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    DHFR cassette
    

    Use

    Gateway cloning

    Mutation

    pyrimethamine resistant

    Promoter

    DHFR

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL30009

    Plasmid

    #50301

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO HIS DNAJB2a

    Plasmid

    #19550

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICSL50010

    Plasmid

    #50310

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal Myc tag (4x Myc)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH41421

    Plasmid

    #50339

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. MXS_CMV::rtTA3-bGHpA

    Plasmid

    #62445

    Purpose

    MXS_chaining vector with CMV::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with CMV promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB700 lenti gRNA Puro

    Plasmid

    #167911

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-Lb-crRNA

    Plasmid

    #78957

    Purpose

    Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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