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Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterARG8Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterGNP1Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_C109A_Y107C
Plasmid#190174PurposeFor expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathwayDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationchanged Cys109 to alanine, changed tyr107 to cyst…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2S31A
Plasmid#174332Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-NKD2S31A
Plasmid#174331Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsMycHisExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC030.306
Plasmid#171151PurposeMevalonate production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mvaES
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MEK-S216D, S220E
Plasmid#107522PurposeConstitutive activation of FGF/MAPK pathway by MEK-S216D, S220E mutantDepositorInsertMEK
UseTagsExpressionMutationchanged Serine 216 to Aspartic Acid and Serine 22…PromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMV-TRP1
Plasmid#65335Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUsDepositorInsertTRP1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 EE Jip1b
Plasmid#52127Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsEEExpressionMammalianMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tadh1
Plasmid#127723PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpre3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tdpp1
Plasmid#127725PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Phta1-ITE-Tvma2
Plasmid#127726PurposeYeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPhta1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeft2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pfba1p-ACO-Tgpm1
Plasmid#127731PurposeYeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pykt6-ACO-Tprm9
Plasmid#127732PurposeYeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPykt6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Ptef1-ACO-Tyol036w
Plasmid#127733PurposeYeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsptdh3Available sinceAvailabilityAcademic Institutions and Nonprofits only