We narrowed to 143,240 results for: ins

1. GFP-MuTMC2truncated

   Plasmid

   #154909

   Purpose

   For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-MuTMC2truncated
   

   Use

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-179 and 691-716

   Promoter

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. DYNLT1

   Plasmid

   #132528

   Purpose

   Human TCTEX-1, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLT1 (DYNLT1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-ChRger1-TS-EYFP

   Plasmid

   #127244

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX Eat2-SH2

   Plasmid

   #46423

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Eat2 (SH2D1B Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-132

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

   Plasmid

   #87202

   Purpose

   To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Residues from 90-107 are inserted.

   Promoter

   CMV

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

   Plasmid

   #138989

   Purpose

   IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX PLCg1(N)-SH2

   Plasmid

   #46470

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(N)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 539-664

   Promoter

   Tac

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACUH-GFP1-10 sec

   Plasmid

   #172062

   Purpose

   UAS vector to express GFP1-10 in the ER lumen

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP1-10 sec
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-hygro-hPGAP3-3HA

   Plasmid

   #50373

   Purpose

   Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-mTAZ-WW (86)

    Plasmid

    #40915

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ 14-3-3 binding site and WW domain (…

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. C. difficile 16S toehold switch sensor

    Plasmid

    #110704

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    C. difficile 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

    Plasmid

    #131618

    Purpose

    Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM]
    NeoR[traF]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH028

    Plasmid

    #80393

    Purpose

    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    

    Use

    Synthetic Biology

    Tags

    Fused by GS linkers to 4 SH3 domains

    Expression

    Bacterial

    Mutation

    Glycine 448 changed to Alanine, makes it primaril…

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Brk-SH2

    Plasmid

    #46411

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Brk (PTK6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 74-176

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-RlACR_477-EYFP

    Plasmid

    #103771

    Purpose

    Anion channelrhodopsin from Rhodomonas lens (RHODO) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R1ACR_477
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pr2-deGFP-MGapt

    Plasmid

    #67736

    Purpose

    OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr2

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pr1-deGFP-MGapt

    Plasmid

    #67735

    Purpose

    OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr1

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Py2087ACR1

    Plasmid

    #153032

    Purpose

    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

    Insert

    Py2087ACR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX SHF-SH2

    Plasmid

    #46491

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHF (SHF Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 317-423

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX SHE-SH2

    Plasmid

    #46490

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHE
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 382-492

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge