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pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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M62 pPET28a-TEV-human beta cat domain N(1-137)

Plasmid

#17203

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Tags

HIS

Expression

Bacterial

Mutation

amino-terminal residues 1-137

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDESTsvaw

Plasmid

#32318

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

ccdB

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag RIOK1

Plasmid

#20543

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RIOK1 (RIOK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PBK

Plasmid

#20558

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PBK (PBK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TEC

Plasmid

#20649

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TEC (TEC Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag NADK

Plasmid

#20415

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NADK (NADK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CKMT1A

Plasmid

#20459

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKMT1A (CKMT1A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CLK1

Plasmid

#20463

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CLK1 (CLK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
3xflag-APEX2-LRRK2

Plasmid

#164622

Purpose

Expresses LRRK2 tagged with APEX2 and 3xFLAG

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

LRRK2 (LRRK2 Human)

Tags

3xFLAG and APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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HC87 pEYFPN1 anti-Homer1 nanobody

Plasmid

#135223

Purpose

Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))

Tags

6xHis-HA-EYFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1 (1-599)

Plasmid

#127461

Purpose

Expressing Flag-tagged human MCPIP1 full lengh protein in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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TagBFP2-INPP4BC842A-CAAX

Plasmid

#116858

Purpose

inactive plasma membrane targetted PI(3,4)P2 phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

HRAS(172-189) and TagBFP2

Expression

Mammalian

Mutation

catalytic cysteine 842 changed to alanine

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSELECT-HA-mFOXO1

Plasmid

#83308

Purpose

Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection marker

Depositor

Steven Abcouwer

Article

Kong et al Exp Eye Res. 2016 Aug 7;151:82-95. doi:

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

hEF1/HTLV prom

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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PCS4 3XFLAG-PPARgamma2

Plasmid

#78770

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
PhyB-mCherry-NLS

Plasmid

#51571

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

NLS and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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tetO-SOX9

Plasmid

#170703

Purpose

doxycycline-inducible overexpression of SOX9

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SOX9 (SOX9 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pdx1-mTQ2-P2A-FlpO-PA

Plasmid

#67279

Purpose

Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

Depositor

Jannik Elverløv-Jakobsen

Inserts

mTQ2=mturquoise2 blue fluorescent gene
FlpO recombinase

Tags

linked to FlpO through an P2A ribosomal skipping …

Expression

Mammalian

Promoter

pdx

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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p38 WT O/E (MAPK14)-pcw107-V5

Plasmid

#64624

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAPK14 (transcript variant 2) (MAPK14 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-puro GFP-UVRAG

Plasmid

#38267

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV radiation resistance-associated gene protein (UVRAG Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only