We narrowed to 146,921 results for: ins;

1. IFI16-(192-393)-HinA (pcDNA3-FLAG)

   Plasmid

   #35062

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16-(192-393)-HinA (IFI16 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   Contains HinA domain: aa 192-393

   Promoter

   CMV

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pBabe-PI(WT)-Src(YF)-mCherry

   Plasmid

   #87357

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src isoform 2 (Src Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Y535F

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. IFI16-(515-710)-HinB (pcDNA3-FLAG)

   Plasmid

   #35063

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16-(515-710)-HinB (IFI16 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains HinB domain: aa 515-710

   Promoter

   CMV

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

4. 2XMyc-LRRK2-WD40

   Plasmid

   #25073

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Contains only the WD40 domain aa 2155-2527

   Promoter

   Available Since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

5. AICSDP-83:EZH2-mEGFP

   Plasmid

   #164499

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2

   Depositor

   Allen Institute for Cell Science

   Insert

   EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. mCherry-Myo10-∆PH

   Plasmid

   #139781

   Purpose

   Detecting Myo10-∆PH by fluorescence microscopy

   Depositor

   Staffan Stromblad

   Article

   Plantard et al J Cell Sci. 2010 Oct 15;123(Pt 20):3525-

   Insert

   Myosin X (MYO10 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Deletion of 3PH domains

   Promoter

   CMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTH PKC zeta

   Plasmid

   #8414

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Mouse Protein Kinase C zeta (Prkcz Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

8. His-ZZ-TEV-SNAPf-BICD2N(1-400)

   Plasmid

   #111857

   Purpose

   N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICD2N(1-400)
   

   Use

   Tags

   His-tag, SNAPf-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   Optimised for Sf9 expression

   Promoter

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACE-GIRK4-S

   Plasmid

   #172425

   Purpose

   Expression of full-length, human GIRK4 with C-terminal StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK4 (KCNJ5 Human)
   

   Use

   Tags

   Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEAK-bKal7

    Plasmid

    #145800

    Purpose

    expresses Kalirin-7 with the b-front in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

    Insert

    bKalirin-7 (Kalrn Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EIF2a

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFUGW-RapACR-EYFP

    Plasmid

    #111075

    Purpose

    Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNP1 tdTomato toehold switch

    Plasmid

    #107354

    Purpose

    Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 5 + tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-PI(WT)-Src(KD)-mCherry

    Plasmid

    #87358

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    D388R

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX SHIP2-SH2

    Plasmid

    #46493

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP2
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 8-131

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJRoC30_VP5H

    Plasmid

    #188061

    Purpose

    PROSS-designed versatile peroxidase 5H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Pleurotus ostreatus
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    43 PROSS mutations, described in publication

    Promoter

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 2XMyc-LRRK2-COR

    Plasmid

    #25069

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the COR domain aa1408-1850

    Promoter

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pGen2.1-redCALWY-4

    Plasmid

    #50738

    Purpose

    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-4 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUGW-RapACR_T111C-EYFP

    Plasmid

    #119195

    Purpose

    Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    changed threonine 111 to cysteine

    Promoter

    Ubiquitin

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28 sf:mid.11

    Plasmid

    #191921

    Purpose

    Encodes a GFP design, sf:mid.11

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.11
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69M Y145F T167I L220V V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 sf:mid.7

    Plasmid

    #191933

    Purpose

    Encodes a GFP design, sf:mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.7
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge