We narrowed to 5,602 results for: pCas

1. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-U6-sgGAA

   Plasmid

   #232724

   Purpose

   Cas9 guide RNA targeting GAA repeats for A-to-G base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting GAA repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. kiCAP-AAV-BI-hTFR1-2

   Plasmid

   #218797

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SGyA

   Plasmid

   #160292

   Purpose

   Drives expression of Cas9 and a TdTomato fluorescent marker

   Depositor

   Omar Akbari

   Article

   Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

   Inserts

   Vas promoter
   hSpCas9
   tdTomato
   CTCF insulator
   Gypsy insulator
   Left homology arm Y chromosome
   Right homology arm Y chromosome
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE7.10-F148A

   Plasmid

   #132946

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   ABE7.10(F148A)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   ABE7.10(F148A)

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TadA-NG-CBE

   Plasmid

   #227420

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   TadA-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-COX8A

   Plasmid

   #227308

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of COX8A (COX8A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U67

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BLBP-GFP

    Plasmid

    #63174

    Purpose

    Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

    Depositor

    Bart De Strooper

    Article

    Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

    Insert

    BLBP (Fabp7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    1700bp upstream of BLBP gene

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

13. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid

    #104567

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    

    Use

    CRISPR

    Tags

    NLS-3xHA-VPR

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH-nCas9-PPE-V2

    Plasmid

    #170131

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1, OsU3

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEX-PK-hLC3∆G

    Plasmid

    #61459

    Purpose

    Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy

    Depositor

    Isei Tanida

    Article

    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

    Insert

    PK-hLC3∆G (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BLBP-mCherry

    Plasmid

    #63721

    Purpose

    Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

    Depositor

    Bart De Strooper

    Article

    Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

    Insert

    BLBP (Fabp7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1700 bp upstream of BLBP gene cloned in CAG-mCher…

    Promoter

    1700bp upstream of BLBP gene

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid

    #104571

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    

    Use

    CRISPR

    Tags

    NLS-3xHA-3xFLAG-4xDmrA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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