We narrowed to 15,827 results for: grna

1. pSJ106-2x

   Plasmid

   #208860

   Purpose

   Expresses SoxS activation domain and gRNA targeting two copies of 106 region within the synthetic promoter in bacterial cell

   Depositor

   Marc Erhardt

   Article

   Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

   Insert

   sgRNA 106-2x_mRFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CRISPR/dCas9-responsive synthetic promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

   Plasmid

   #99696

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-dErbB4

   Plasmid

   #197357

   Purpose

   A knock-out vector for dog ErbB4.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-dErbB1

   Plasmid

   #197354

   Purpose

   A knock-out vector for dog ErbB1.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-dErbB2

   Plasmid

   #197355

   Purpose

   A knock-out vector for dog ErbB2.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-dErbB3

   Plasmid

   #197356

   Purpose

   A knock-out vector for dog ErbB3.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORE303N_CEN12Fuzzy3MYB

   Plasmid

   #194440

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYB

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA array targeting CEN1, Fuzzy3MYB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MtU6

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORE303N_CEN1

   Plasmid

   #194439

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA targeting CEN1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MtU6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. rtTA-sg-mGreenLantern332

   Plasmid

   #188483

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   PB_rtTA_BsmBI

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. rtTA-sg-CMV51

    Plasmid

    #188484

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. rtTA-sg-mGreenLantern/EGFP

    Plasmid

    #188482

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pORANGE Tubb3-2xGFP KI

    Plasmid

    #183443

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 2xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOC4 Kcnn2-smFP-myc KI

    Plasmid

    #183438

    Purpose

    FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP myc donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOC2 GFP-Mpp2 KI

    Plasmid

    #183434

    Purpose

    CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOC5 smFP-HA-Cacna1e KI

    Plasmid

    #183441

    Purpose

    FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOC2 Gria1-Halo KI

    Plasmid

    #183433

    Purpose

    CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pORANGE Tubb3-3xGFP KI

    Plasmid

    #183444

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 3xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOC2 GFP-Actb KI

    Plasmid

    #183429

    Purpose

    CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOC2 Gria1-GFP KI

    Plasmid

    #183430

    Purpose

    CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEDJ-22

    Plasmid

    #140904

    Purpose

    MarkerFree plasmid for integration of pADH1-PCP-Mxi1 into site X-4

    Depositor

    Michael Jensen

    Article

    Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

    Insert

    PCP-Mxi1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    ADH1

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLY86

    Plasmid

    #130949

    Purpose

    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEA2-WTmis2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plux2

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB6637

    Plasmid

    #106164

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRRNA-G10-R20

    Plasmid

    #101819

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRRNA-G11-R0

    Plasmid

    #101805

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSpCas9 (BB)-2A-GFP gamma-tubulin sg

    Plasmid

    #104437

    Purpose

    Knockdown the expression of gamma-tubulin in human cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin sgRNA
    

    Use

    Tags

    The gamma-tubulin sgRNA is coexpressed with 3xFLA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCRRNA-G14-R20

    Plasmid

    #101821

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRRNA-G10-R11

    Plasmid

    #101809

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRRNA-G0-R11

    Plasmid

    #101808

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    David Bikard, Sven van Teeffelen

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge