We narrowed to 13,645 results for: sequence
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Plasmid#216540PurposeThis retroviral plasmid expresses the human wild type PTK2DepositorAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
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BFP-KDEL
Plasmid#49150Purposevisualization of the ERDepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLHKII-pGFPN3
Plasmid#21920DepositorInsertFull length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)
TagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …Available SinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralExpressionMammalianAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LXRalpha_pcDNA3.1
Plasmid#177621PurposeFull-length human LXR alpha cDNA sequence in pcDNA3.1+DepositorAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585
Plasmid#174401PurposeS. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.DepositorInsertsarA
ExpressionBacterialAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pP2A-mCherry-N1
Plasmid#84329PurposeEncodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherryDepositorInsertP2A-mCherry
ExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-1
Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionExpressionWormPromoterT7Available SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABCB10-ChR2-eYFP
Plasmid#206037PurposeExpress ChR2 in mitochondriaDepositorInsertChR2
TagsABCB10 mitochondrial leading sequence and EYFPExpressionMammalianAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2-sgControl
Plasmid#230080PurposeCrispr knock out controlDepositorInsertnon-specific sequence control
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MBII85-5'SS mut
Plasmid#67631PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.DepositorInsertMBII85 snoRNA
ExpressionMammalianMutationmutation in 5'ssAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
ExpressionBacterialPromoterPF6Available SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_pET28
Plasmid#34705DepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationEncodes residues 43–246, corresponding to full-le…PromoterT7Available SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTagBFP2-WPRE
Plasmid#193339PurposeAAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseAAVExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC scAB-GFP
Plasmid#104998PurposeLentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labelingDepositorInsertsingle chain Antibody against GCN4
UseLentiviralTagsGB1, HA tag, and sfGFPExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mios in pRK5
Plasmid#154212PurposeN-terminal HA-tagged Mios cDNA expression vector for transfectionsDepositorInsertMios
TagsHA tagExpressionMammalianMutationcDNA sequence has been codon optimized using huma…PromoterCMVAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FRB-GFP11
Plasmid#181863PurposePlasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.DepositorInsertLyn-FRB-GFP11
TagsGFP11 and N-terminal targeting sequence from Lyn …ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterP(rhaB)Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-ExRai-CKAR2
Plasmid#236098PurposePlasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertLyn-ExRai-CKAR2
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
Tagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastPromoterPGK1Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cytb5
Plasmid#182579PurposeExpresses mCherry targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertmCherry-Cytb5
TagsCytb5ExpressionMammalianAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
EFS-GFP
Plasmid#110834Purposepositive control for GFP expressionDepositorInsertelongation factor 1alpha binding sequence (EFS) at GFP promoter region
UseLentiviralExpressionMammalianAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pER-eZinCh-2
Plasmid#70009Purposeexpresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cellsDepositorInsertER-eZinCh-2
TagsKDEL sequence and PPI signalExpressionMammalianPromoterCMVAvailable SinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcSTH
Plasmid#211923PurposeThis plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.DepositorInsertEcSTH
UseLentiviral and Synthetic BiologyTags3xFLAGAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSin Helper
Plasmid#127694PurposePlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Glycoproteins
UseSynthetic BiologyAvailable SinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmScarlet-I_peroxisome_C1
Plasmid#85065PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
TagsmScarlet-iExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only