We narrowed to 19,638 results for: ire;
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Plasmid#90426Purposeselectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)DepositorInsertSf3b1 - left and right homology arms (Sf3b1 Mouse)
UseHomology-directed repair vectorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishPromoterUASAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-CAAX-B-actin
Plasmid#117816PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsCAAXAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-TagRFP-B-actin
Plasmid#117806PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsNLSAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-B-actin
Plasmid#117812PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-Y117C
Plasmid#110216PurposeExpression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsFlagExpressionMammalianMutationY117C, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-Y117C-FL
Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-TR-TTHA
Plasmid#232479PurposeTetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' endDepositorInsertThermus thermophilus HB8
UseTransposon systemTagsDYKDDDDK (FLAG-tag)ExpressionMammalianPromoterCMV-TetOAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SFFV-RELB-Brd 299
Plasmid#219235PurposeTranscription factor RELB with a specific barcode assigned.DepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-Sox17-p2A-Erg-t2A-GFP
Plasmid#222496Purposemouse fibroblast-to-endothelial cell direct reprogrammingDepositorAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-mVenus-p27K−
Plasmid#176651PurposeIt encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).DepositorInsertmVenus-p27K- (Cdkn1b Mouse)
UseLentiviralTagsmVenusMutationMutant K- (lacks affinity to Cdk)Available SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD
Plasmid#226282PurposeTetracycline-inducible expression of Isl1 and Lhx3 for direct reprogramming of canine fibroblasts to induced-motor neuronsDepositorUseLentiviralTagsIsl1 and Lhx3 linked via T2AExpressionMammalianAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2_2A_Sox11-PURO
Plasmid#226284PurposeTetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neuronsDepositorUseLentiviralTagsNGN2 and SOX11 linked via T2AExpressionMammalianAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-mSNCA-NE
Plasmid#102364PurposeLentiviral overexpression of PA-mCherry synuclein alpha fusionDepositorInsertsUseLentiviralTagsNEExpressionMammalianPromoterEF-1aAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc-EGFP-cre puro
Plasmid#67503PurposeConstitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cellsDepositorInsertluciferase-EGFP-cre
UseCre/Lox, Lentiviral, and Luciferase ; Fluorescent…ExpressionMammalianPromoterCAGAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.1248
Plasmid#19761DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiExpressionMammalianAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRExpressionMammalianPromoterU6Available SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.sh.beta-catenin.2279
Plasmid#19762DepositorInsertsmall hairpin RNA against beta-catenin (CTNNB1 Human)
UseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
NGFR P210
Plasmid#27486DepositorUseRetroviralTagsIRES and NFGRExpressionMammalianMutationContains the "complete" bcr/abl fusionAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSIN-hSNCA-NE
Plasmid#102366PurposeLentiviral overexpression of synuclein alphaDepositorInsertsynuclein alpha (SNCA Human)
UseLentiviralTagsNEExpressionMammalianMutationA silent mutation (333bp downstream of ATG) was i…PromoterEF-1aAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only