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We narrowed to 156,064 results for: ins

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Showing: 8041 - 8070 of 156064 results

  5. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

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  9. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid
    #197504
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197504-plasmid-map-sequence-id-386610

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  10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  13. pADC10-SETXhel

    Plasmid
    #193056
    Purpose
    Baculovirus expression of human Senataxin helicase domain for protein production
    Depositor
    Andrew Deans
    Article
    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:
    Insert
    Senataxin (SETX Human)
    Tags
    Flag
    Expression
    Insect and Mammalian
    Mutation
    codon optimized for insect cell expression
    Promoter
    hybrid p10 (insect) and CMV (human) expression
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    193056-plasmid-map-sequence-id-381199

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  14. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  17. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

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  21. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid
    #231552
    Purpose
    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    F-tractin (Itpka Rat)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231552-plasmid-map-sequence-id-459701

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  24. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

    Plasmid
    #216162
    Purpose
    Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216162-plasmid-map-sequence-id-437755

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Showing: 8041 - 8070 of 156064 results