1,878 results

1. pJK2067

   Plasmid

   #171845

   Purpose

   C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

   Depositor

   Judith Kimble

   Article

   Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

   Insert

   his-58, eGFP, mCherry
   

   Tags

   N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

   Expression

   Worm

   Mutation

   boxB wild type and hairpin mutant

   Promoter

   mex-5 promoter

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMNK55 - piRNAi 21ur-1224 - cku-80

   Plasmid

   #159820

   Purpose

   piRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombination

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   cku-80 piRNAi 21ur-1224
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMNK54 - piRNAi 21ur-1224 - him-5

   Plasmid

   #159818

   Purpose

   piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   him-5 piRNAi 21ur-1224
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMNK56 - piRNAi 21ur-1224 - him-8

   Plasmid

   #159819

   Purpose

   piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

   Depositor

   Christian Froekjaer-Jensen

   Article

   Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

   Insert

   him-8 piRNAi 21ur-1224
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAP685-2

   Plasmid

   #99490

   Purpose

   TEV::eGFP::ER::6xHis::linker::3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::eGFP::RE::6Xhis::linker::3XFlag
   

   Expression

   Bacterial

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOX-Shaw2

   Plasmid

   #16114

   Depositor

   Larry Salkoff

   Article

   Wei et al Neuropharmacology. 1996 . 35(7):805-29.

   Insert

   Voltage-gated K+ channel (egl-36 Nematode)
   

   Use

   Xenopus oocyte expression

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pJW1927

   Plasmid

   #163093

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1886

   Plasmid

   #154333

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOX-twk18

   Plasmid

   #16430

   Depositor

   Larry Salkoff

   Article

   Kunkel et al J Neurosci. 2000 Oct 15. 20(20):7517-24.

   Insert

   Tandem pore domain K+ channel twk-18 (twk-18 Nematode)
   

   Use

   Xenopus oocyte expression

   Mutation

   N/A

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pSEM234 - pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]

    Plasmid

    #159795

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    pEXP[Pmlc-1 | mCherry(2xNLS) | cbr-tbb-2 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KVS1-HA

    Plasmid

    #113061

    Purpose

    Expresses worm KVS-1 in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

    Insert

    K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ2214 - Intron GG1 - 900 bp

    Plasmid

    #159880

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 900 bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCZGY3195

    Plasmid

    #172695

    Purpose

    Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    Punc-25
    

    Tags

    GFP 1-10

    Expression

    Worm

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV myc DAF-12

    Plasmid

    #32566

    Depositor

    Al Fisher

    Article

    Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

    Insert

    DAF-12 (daf-12 Nematode)
    

    Tags

    myc epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSR35

    Plasmid

    #69256

    Purpose

    phsp16.41::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    hsp16.41

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGC158

    Plasmid

    #19620

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plag-2::FLP::let-858(3’)
    

    Use

    Flp/frt

    Expression

    Worm

    Promoter

    lag-2 promoter

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS2-his-24::mNeptune

    Plasmid

    #124332

    Purpose

    Expressing Nuclear Localized mNeptune

    Depositor

    Andrew Leifer

    Insert

    mNeptune (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBN281

    Plasmid

    #86726

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Plin-31::FLP D5
    

    Expression

    Worm

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD316

    Plasmid

    #137020

    Purpose

    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    mTurq2-GLO^SEC^2xHA + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans GLO-optimized mTurq2

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBN258

    Plasmid

    #86721

    Purpose

    Tissue-specific FLP expression plasmid for C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    Punc-119::FLP D5
    

    Expression

    Worm

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFB-GluCl_cryst

    Plasmid

    #31488

    Depositor

    Eric Gouaux

    Article

    Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

    Insert

    C. elegans glutamate-gated chloride channel alpha, crystallized construct
    

    Tags

    8x-His

    Expression

    Insect

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pGC240

    Plasmid

    #19625

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plim-7

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pISlin41w(s) (lin-41 microRNA wt reporter)

    Plasmid

    #11906

    Depositor

    David Bartel

    Article

    Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

    Insert

    lin-41 miRNA target sequence (lin-41 Nematode)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMLS297

    Plasmid

    #73737

    Purpose

    SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    STOP codon + let-858 3'-UTR
    

    Use

    CRISPR

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCM5.34

    Plasmid

    #21390

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    fbf-2 3'utr in pDONRP2RP3
    

    Use

    Gateway

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. pNM3810

    Plasmid

    #154119

    Purpose

    mec-4 promoter driving QF in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::QF::act-4 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pOD2049-csnControl

    Plasmid

    #100007

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAS2-his-24::tagBFP

    Plasmid

    #124320

    Purpose

    Expressing Nuclear Localized tagBFP

    Depositor

    Andrew Leifer

    Insert

    tagBFP (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJW4.21

    Plasmid

    #62875

    Purpose

    Bacterial expression of MBP-MEG-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MEG-1
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCFJ914

    Plasmid

    #44489

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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