Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,793 results for: plasmid for e coli

Clear keywords
Showing: 1041 - 1080 of 1793 results

  17. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available Since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

    Enlarge

  18. pET28-Strep-TEV-Nsp14

    Plasmid
    #154955
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    Strep-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154955-plasmid-map-sequence-id-307850

    Enlarge

  19. pET28-6His-SUMO-Nsp10

    Plasmid
    #154959
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154959-plasmid-map-sequence-id-307848

    Enlarge

  20. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    6His-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

    Enlarge

  21. pET22-Nsp10-TEV-6His

    Plasmid
    #154957
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    Tags
    TEV-6His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154957-plasmid-map-sequence-id-306327

    Enlarge

  22. pET28-Nsp14-TEV-6His

    Plasmid
    #154952
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    Tags
    TEV-6His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154952-plasmid-map-sequence-id-307820

    Enlarge

  23. pET28-Nsp14-TEV-Strep

    Plasmid
    #154953
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    Tags
    TEV-Strep
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154953-plasmid-map-sequence-id-307852

    Enlarge

  24. pET28-6His-SUMO-Nsp14

    Plasmid
    #154956
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154956-plasmid-map-sequence-id-307845

    Enlarge

  25. pET22-6His-SUMO-Nsp10

    Plasmid
    #154960
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154960-plasmid-map-sequence-id-306350

    Enlarge

  26. pET22-Strep-TEV-Nsp10

    Plasmid
    #154958
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    Strep-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154958-plasmid-map-sequence-id-306337

    Enlarge

  36. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

    Enlarge

  40. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40786-plasmid-map-sequence-id-68901

    Enlarge

Showing: 1041 - 1080 of 1793 results