We narrowed to 2,481 results for: D10
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Plasmid#135338Purpose3xFLAG 2xVP64 SadCas9 for single guide cloningDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSadCas9
Tags3xFLAG-VP64-SV40 NLS and NLS-VP64ExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE-zCas9
Plasmid#101739Purposeexpression of an optimized Cas9 for base-editing in zebrafishDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrAPOBEC1-XTEN-zCas9-UGI-NLS
UseCRISPRExpressionMammalianMutationD10APromoterCMVAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLL7 lenti-dcas9-3xflag-blast
Plasmid#112133Purposelenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)DepositorInsertdCas9(D10A, N863A)-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A mutants in Cas9PromoterEF1aAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LAMP-mCherry-CRY2
Plasmid#102249PurposeExpresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-OptoSTIM1(Cry2(D387A))
Plasmid#70160PurposeLight-insensitive mutant of OptoSTIM1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOptoSTIM1(Cry2(D387A)) (STIM1 Human, Mustard Weed)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LLP457 pGK-dCas9-Suntag-BFP
Plasmid#100957PurposeBPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tagDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHA and BFPExpressionMammalianMutationD10A, H840A for dCas9Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-hA3G-BE3
Plasmid#113415PurposeExpresses hA3G-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-dCas9
Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
Tags3XFLAG, mCherry, and pHluorinExpressionMammalianPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNAHygro(+)LAMP2B
Plasmid#86029PurposeExpresses LAMP2B in Mammalian cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLysosome-associated membrane protein 2b (LAMP2 Human)
ExpressionMammalianPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Target-AID (pRZ762)
Plasmid#131300PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTen-Eleven Translocation 1 (Tet1 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-xFNLS-P2A-Puro
Plasmid#110872PurposeLentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)DepositorInsertxFNLS(3.7)
UseLentiviralTags3X FLAGMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEX-SP-CFP-STIM1(23-685)
Plasmid#18858DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStromal interaction molecule 1 (STIM1 Human)
TagsCFPExpressionMammalianMutationCFP inserted between residues 1-23 (signal peptid…Available sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDF-His-mTET1CD
Plasmid#81053Purposebacterial expression of the catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by