We narrowed to 1,037 results for: David Sabatini

1. BCL2-pcw107-V5

   Plasmid

   #64630

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   BCL2 (transcript variant alpha) (BCL2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pK RagA-RagC

   Plasmid

   #99649

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   10x His tag, Poly arginine linker, and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-YAP2 (8SA)-pcw107

   Plasmid

   #64637

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   YAP2 (transcript variant 3) (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   8SA*

   Promoter

   PGK

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRK5 Flag-RagC(L91P)

   Plasmid

   #99726

   Purpose

   Mammalian expression of Flag-RagC(L91P)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   RagC (RRAGC )
   

   Use

   Tags

   Flag

   Expression

   Mutation

   L91P

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5 Flag-RagC(K84T)

   Plasmid

   #99727

   Purpose

   Mammalian expression of Flag-RagC(K84T)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   RagC (RRAGC )
   

   Use

   Tags

   Flag

   Expression

   Mutation

   K84T

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5 Flag-RagA(R37P)

   Plasmid

   #99717

   Purpose

   Mammalian expression of Flag-RagA(R37P)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Insert

   RagA (RRAGA )
   

   Use

   Tags

   Flag

   Expression

   Mutation

   R37P

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. [pSK497] pLJC5 Flag-Depdc5(AB)

   Plasmid

   #109341

   Purpose

   Lenti-viral expression of Flag-tagged Depdc5 mutant AB

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW57.1 tTA SDHA

   Plasmid

   #177976

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   SDHA (SDHA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to SDHA sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETDuet RagA-RagC(L91P)

   Plasmid

   #99647

   Purpose

   Bacterial expression of RagA-RagC(L91P)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L91P

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5 HA-RagC(K84T)

    Plasmid

    #99722

    Purpose

    Mammalian expression of HA-RagC(K84T)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    RagC (RRAGC )
    

    Use

    Tags

    HA

    Expression

    Mutation

    K84T

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5 HA-RagC(L91P)

    Plasmid

    #99721

    Purpose

    Mammalian expression of HA-RagC(L91P)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    RagC (RRAGC )
    

    Use

    Tags

    HA

    Expression

    Mutation

    L91P

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5 HA-RagA(R37P)

    Plasmid

    #99713

    Purpose

    Mammalian expression of HA-RagA(R37P)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    RagA (RRAGA )
    

    Use

    Tags

    HA

    Expression

    Mutation

    R37P

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RalA (G23V)( full )-pcw107-V5

    Plasmid

    #64645

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    G23V

    Promoter

    PGK

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pK RagA-RagC(Q120L)

    Plasmid

    #99701

    Purpose

    Bacterial expression of RagA-RagC(Q120L)

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    Q120L

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HcRed-pcw107

    Plasmid

    #64646

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

16. RalA (G23V)( full )-pcw107

    Plasmid

    #64644

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G23V

    Promoter

    PGK

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLentiCRISPRv1_COX4

    Plasmid

    #177983

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting COX4

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting COX4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmxs_COX4

    Plasmid

    #177979

    Purpose

    Mammalian expression of COX4 cDNA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to COX4 sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge