We narrowed to 2,087 results for: Endog

Showing: 521 - 540 of 2087 results

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pEGFP_sfCherry2(11)_HP1

Plasmid

#82607

Purpose

Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_HP1b

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only
pGEMHE-H2B-mEGFP

Plasmid

#105528

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

H2B (H2BC11 Human)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-ATG16L1 HRD

Plasmid

#207537

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F2

Plasmid

#184167

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F1

Plasmid

#184166

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Tornado-RNA sensor for MALAT-1

Plasmid

#185406

Purpose

circular RNA-scaffolded sensing modules for MALAT-1

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Expression

Mammalian

Mutation

WT

Promoter

U6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-PL Key

Plasmid

#216503

Purpose

Ras activity dependent proximity labeler, Key only, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-PL Key

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-PL Cage

Plasmid

#216502

Purpose

Ras activity dependent proximity labeler, Cage only, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-PL Cage

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-PGK-Blast

Plasmid

#227265

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-53BP1 HRD

Plasmid

#207087

Purpose

Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRS423-GPD-wrmScarlet1-10-CYC1 terminator

Plasmid

#158584

Purpose

Expresses wrmScarlet1-10 in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Expression

Yeast

Promoter

GPD

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-7xGFP11

Plasmid

#138397

Purpose

RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

7xGFP11

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMLM3705

Plasmid

#47754

Purpose

Expresses mammalian cell codon-optimized dCas9-VP64

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

codon optimized dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9 (catalytically i…

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Ub(L40)

Plasmid

#52892

Purpose

Aedes aegypti ubiquitin promoter driving firefly luciferase

Depositor

Zach Adelman

Article

Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

Insert

ubiquitin promoter

Use

Luciferase

Promoter

Ae aegypti ubiquitin

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pFSW FLEx mCherry-KASH

Plasmid

#139652

Purpose

Conditional expression of mCherry-KASH

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry-KASH

Expression

Mammalian

Promoter

Synapsin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-TJP1

Plasmid

#227299

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TJP1 (TJP1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Lv EF1a SS18-Frb PGK Puro

Plasmid

#102811

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

SS18-Frb

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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